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Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation

Seminal plasma (SP), particularly SP exosomes (sExos), alters with age and can affect female mouse uterine immune microenvironment. However, the relationship between fertility decline in reproductively older males, and SP and sExos age-related changes, which may compromise the uterine immune microen...

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Autores principales: Wang, Dandan, Jueraitetibaike, Kadiliya, Tang, Ting, Wang, Yanbo, Jing, Jun, Xue, Tongmin, Ma, Jinzhao, Cao, Siyuan, Lin, Ying, Li, Xiaoyan, Ma, Rujun, Chen, Xi, Yao, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546305/
https://www.ncbi.nlm.nih.gov/pubmed/34712227
http://dx.doi.org/10.3389/fimmu.2021.723409
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author Wang, Dandan
Jueraitetibaike, Kadiliya
Tang, Ting
Wang, Yanbo
Jing, Jun
Xue, Tongmin
Ma, Jinzhao
Cao, Siyuan
Lin, Ying
Li, Xiaoyan
Ma, Rujun
Chen, Xi
Yao, Bing
author_facet Wang, Dandan
Jueraitetibaike, Kadiliya
Tang, Ting
Wang, Yanbo
Jing, Jun
Xue, Tongmin
Ma, Jinzhao
Cao, Siyuan
Lin, Ying
Li, Xiaoyan
Ma, Rujun
Chen, Xi
Yao, Bing
author_sort Wang, Dandan
collection PubMed
description Seminal plasma (SP), particularly SP exosomes (sExos), alters with age and can affect female mouse uterine immune microenvironment. However, the relationship between fertility decline in reproductively older males, and SP and sExos age-related changes, which may compromise the uterine immune microenvironment, remains unclear. The present study demonstrated that the implantation rate of female mice treated with SP from reproductively older male mice (aged-SP group) was lower than that of those treated with SP from younger male mice (young-SP group). RNA-sequencing analysis revealed altered levels of dendritic cell (DC)-related cytokines and chemokines in the uteri of the former group compared with those of the latter group. In vivo and in vitro experiments demonstrated a weaker inhibitory effect of aged SP on DC maturation than of young SP upon stimulation. After isolating and characterizing sExos from young and advanced-age male mice, we discovered that insemination of a subset of the aged-SP group with sExos from young male mice partially recovered the implantation rate decline. Additional in vivo and in vitro experiments revealed that sExos extracted from age male mice exerted a similar effect on DC maturation as SP of aged mice, indicating an age-related sExos inhibitory effect. In conclusion, our study demonstrated that age-related alterations of sExos may be partially responsible for lower implantation rates in the aged-SP group compared with those in the young-SP group, which were mediated by uterine immunomodulation. These findings provide new insights for clinical seminal adjuvant therapy.
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spelling pubmed-85463052021-10-27 Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation Wang, Dandan Jueraitetibaike, Kadiliya Tang, Ting Wang, Yanbo Jing, Jun Xue, Tongmin Ma, Jinzhao Cao, Siyuan Lin, Ying Li, Xiaoyan Ma, Rujun Chen, Xi Yao, Bing Front Immunol Immunology Seminal plasma (SP), particularly SP exosomes (sExos), alters with age and can affect female mouse uterine immune microenvironment. However, the relationship between fertility decline in reproductively older males, and SP and sExos age-related changes, which may compromise the uterine immune microenvironment, remains unclear. The present study demonstrated that the implantation rate of female mice treated with SP from reproductively older male mice (aged-SP group) was lower than that of those treated with SP from younger male mice (young-SP group). RNA-sequencing analysis revealed altered levels of dendritic cell (DC)-related cytokines and chemokines in the uteri of the former group compared with those of the latter group. In vivo and in vitro experiments demonstrated a weaker inhibitory effect of aged SP on DC maturation than of young SP upon stimulation. After isolating and characterizing sExos from young and advanced-age male mice, we discovered that insemination of a subset of the aged-SP group with sExos from young male mice partially recovered the implantation rate decline. Additional in vivo and in vitro experiments revealed that sExos extracted from age male mice exerted a similar effect on DC maturation as SP of aged mice, indicating an age-related sExos inhibitory effect. In conclusion, our study demonstrated that age-related alterations of sExos may be partially responsible for lower implantation rates in the aged-SP group compared with those in the young-SP group, which were mediated by uterine immunomodulation. These findings provide new insights for clinical seminal adjuvant therapy. Frontiers Media S.A. 2021-10-12 /pmc/articles/PMC8546305/ /pubmed/34712227 http://dx.doi.org/10.3389/fimmu.2021.723409 Text en Copyright © 2021 Wang, Jueraitetibaike, Tang, Wang, Jing, Xue, Ma, Cao, Lin, Li, Ma, Chen and Yao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wang, Dandan
Jueraitetibaike, Kadiliya
Tang, Ting
Wang, Yanbo
Jing, Jun
Xue, Tongmin
Ma, Jinzhao
Cao, Siyuan
Lin, Ying
Li, Xiaoyan
Ma, Rujun
Chen, Xi
Yao, Bing
Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title_full Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title_fullStr Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title_full_unstemmed Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title_short Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
title_sort seminal plasma and seminal plasma exosomes of aged male mice affect early embryo implantation via immunomodulation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546305/
https://www.ncbi.nlm.nih.gov/pubmed/34712227
http://dx.doi.org/10.3389/fimmu.2021.723409
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