Species-specific PCR assay for the detection of Babesia odocoilei

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 The...

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Autores principales: Burgess, Hilary J., Lockerbie, Betty P., Ayalew, Lisanework E., Dibernardo, Antonia, Hrazdilová, Kristýna, Modry, David, Bollinger, Trent K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546463/
https://www.ncbi.nlm.nih.gov/pubmed/34550025
http://dx.doi.org/10.1177/10406387211032927
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author Burgess, Hilary J.
Lockerbie, Betty P.
Ayalew, Lisanework E.
Dibernardo, Antonia
Hrazdilová, Kristýna
Modry, David
Bollinger, Trent K.
author_facet Burgess, Hilary J.
Lockerbie, Betty P.
Ayalew, Lisanework E.
Dibernardo, Antonia
Hrazdilová, Kristýna
Modry, David
Bollinger, Trent K.
author_sort Burgess, Hilary J.
collection PubMed
description We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
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spelling pubmed-85464632021-12-01 Species-specific PCR assay for the detection of Babesia odocoilei Burgess, Hilary J. Lockerbie, Betty P. Ayalew, Lisanework E. Dibernardo, Antonia Hrazdilová, Kristýna Modry, David Bollinger, Trent K. J Vet Diagn Invest Brief Reports We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei. SAGE Publications 2021-09-22 2021-11 /pmc/articles/PMC8546463/ /pubmed/34550025 http://dx.doi.org/10.1177/10406387211032927 Text en © 2021 The Author(s)
spellingShingle Brief Reports
Burgess, Hilary J.
Lockerbie, Betty P.
Ayalew, Lisanework E.
Dibernardo, Antonia
Hrazdilová, Kristýna
Modry, David
Bollinger, Trent K.
Species-specific PCR assay for the detection of Babesia odocoilei
title Species-specific PCR assay for the detection of Babesia odocoilei
title_full Species-specific PCR assay for the detection of Babesia odocoilei
title_fullStr Species-specific PCR assay for the detection of Babesia odocoilei
title_full_unstemmed Species-specific PCR assay for the detection of Babesia odocoilei
title_short Species-specific PCR assay for the detection of Babesia odocoilei
title_sort species-specific pcr assay for the detection of babesia odocoilei
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546463/
https://www.ncbi.nlm.nih.gov/pubmed/34550025
http://dx.doi.org/10.1177/10406387211032927
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