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A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG

Signal transduction is essential for bacteria to adapt to changing environmental conditions. Among many forms of posttranslational modifications, reversible protein phosphorylation has evolved as a ubiquitous molecular mechanism of protein regulation in response to specific stimuli. The Ser/Thr prot...

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Autores principales: Lisa, María-Natalia, Sogues, Adrià, Barilone, Nathalie, Baumgart, Meike, Gil, Magdalena, Graña, Martín, Durán, Rosario, Biondi, Ricardo M., Bellinzoni, Marco, Bott, Michael, Alzari, Pedro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546545/
https://www.ncbi.nlm.nih.gov/pubmed/34607462
http://dx.doi.org/10.1128/mBio.01717-21
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author Lisa, María-Natalia
Sogues, Adrià
Barilone, Nathalie
Baumgart, Meike
Gil, Magdalena
Graña, Martín
Durán, Rosario
Biondi, Ricardo M.
Bellinzoni, Marco
Bott, Michael
Alzari, Pedro M.
author_facet Lisa, María-Natalia
Sogues, Adrià
Barilone, Nathalie
Baumgart, Meike
Gil, Magdalena
Graña, Martín
Durán, Rosario
Biondi, Ricardo M.
Bellinzoni, Marco
Bott, Michael
Alzari, Pedro M.
author_sort Lisa, María-Natalia
collection PubMed
description Signal transduction is essential for bacteria to adapt to changing environmental conditions. Among many forms of posttranslational modifications, reversible protein phosphorylation has evolved as a ubiquitous molecular mechanism of protein regulation in response to specific stimuli. The Ser/Thr protein kinase PknG modulates the fate of intracellular glutamate by controlling the phosphorylation status of the 2-oxoglutarate dehydrogenase regulator OdhI, a function that is conserved among diverse actinobacteria. PknG has a modular organization characterized by the presence of regulatory domains surrounding the catalytic domain. Here, we present an investigation using in vivo experiments, as well as biochemical and structural methods, of the molecular basis of the regulation of PknG from Corynebacterium glutamicum (CgPknG), in the light of previous knowledge available for the kinase from Mycobacterium tuberculosis (MtbPknG). We found that OdhI phosphorylation by CgPknG is regulated by a conserved mechanism that depends on a C-terminal domain composed of tetratricopeptide repeats (TPRs) essential for metabolic homeostasis. Furthermore, we identified a conserved structural motif that physically connects the TPR domain to a β-hairpin within the flexible N-terminal region that is involved in docking interactions with OdhI. Based on our results and previous reports, we propose a model in which the TPR domain of PknG couples signal detection to the specific phosphorylation of OdhI. Overall, the available data indicate that conserved PknG domains in distant actinobacteria retain their roles in kinase regulation in response to nutrient availability.
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spelling pubmed-85465452021-11-04 A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG Lisa, María-Natalia Sogues, Adrià Barilone, Nathalie Baumgart, Meike Gil, Magdalena Graña, Martín Durán, Rosario Biondi, Ricardo M. Bellinzoni, Marco Bott, Michael Alzari, Pedro M. mBio Research Article Signal transduction is essential for bacteria to adapt to changing environmental conditions. Among many forms of posttranslational modifications, reversible protein phosphorylation has evolved as a ubiquitous molecular mechanism of protein regulation in response to specific stimuli. The Ser/Thr protein kinase PknG modulates the fate of intracellular glutamate by controlling the phosphorylation status of the 2-oxoglutarate dehydrogenase regulator OdhI, a function that is conserved among diverse actinobacteria. PknG has a modular organization characterized by the presence of regulatory domains surrounding the catalytic domain. Here, we present an investigation using in vivo experiments, as well as biochemical and structural methods, of the molecular basis of the regulation of PknG from Corynebacterium glutamicum (CgPknG), in the light of previous knowledge available for the kinase from Mycobacterium tuberculosis (MtbPknG). We found that OdhI phosphorylation by CgPknG is regulated by a conserved mechanism that depends on a C-terminal domain composed of tetratricopeptide repeats (TPRs) essential for metabolic homeostasis. Furthermore, we identified a conserved structural motif that physically connects the TPR domain to a β-hairpin within the flexible N-terminal region that is involved in docking interactions with OdhI. Based on our results and previous reports, we propose a model in which the TPR domain of PknG couples signal detection to the specific phosphorylation of OdhI. Overall, the available data indicate that conserved PknG domains in distant actinobacteria retain their roles in kinase regulation in response to nutrient availability. American Society for Microbiology 2021-10-05 /pmc/articles/PMC8546545/ /pubmed/34607462 http://dx.doi.org/10.1128/mBio.01717-21 Text en Copyright © 2021 Lisa et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Lisa, María-Natalia
Sogues, Adrià
Barilone, Nathalie
Baumgart, Meike
Gil, Magdalena
Graña, Martín
Durán, Rosario
Biondi, Ricardo M.
Bellinzoni, Marco
Bott, Michael
Alzari, Pedro M.
A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title_full A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title_fullStr A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title_full_unstemmed A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title_short A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG
title_sort tetratricopeptide repeat scaffold couples signal detection to odhi phosphorylation in metabolic control by the protein kinase pkng
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546545/
https://www.ncbi.nlm.nih.gov/pubmed/34607462
http://dx.doi.org/10.1128/mBio.01717-21
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