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Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro
BACKGROUND: Cardiac surgery with cardiopulmonary bypass (CPB) is commonly associated with acute kidney injury, and microvascular endothelial inflammation is a potential underlying mechanism. We hypothesized that pro-inflammatory components of plasma from patients who underwent coronary artery bypass...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547679/ https://www.ncbi.nlm.nih.gov/pubmed/34760660 http://dx.doi.org/10.4103/IJCIIS.IJCIIS_197_20 |
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author | Ellermann, Sophie F. L. Scheeren, Thomas W. Jongman, Rianne M. Nordhoff, Katja Schnabel, Christiane L. Molema, Grietje Theilmeier, Gregor Meurs, Matijs Van |
author_facet | Ellermann, Sophie F. L. Scheeren, Thomas W. Jongman, Rianne M. Nordhoff, Katja Schnabel, Christiane L. Molema, Grietje Theilmeier, Gregor Meurs, Matijs Van |
author_sort | Ellermann, Sophie F. |
collection | PubMed |
description | BACKGROUND: Cardiac surgery with cardiopulmonary bypass (CPB) is commonly associated with acute kidney injury, and microvascular endothelial inflammation is a potential underlying mechanism. We hypothesized that pro-inflammatory components of plasma from patients who underwent coronary artery bypass graft surgery with CPB induce endothelial adhesion molecule expression when incorporating altered shear stress in the in vitro model. METHODS: The clinical characteristics and markers of systemic inflammation and kidney injury were analyzed pre and postoperatively in 29 patients undergoing coronary artery bypass grafting with CPB. The effects of tumor necrosis factor (TNF)-α and patient plasma on the expression of endothelial inflammation and adhesion markers were analyzed in vitro. RESULTS: Plasma TNF-α was elevated 6 h postoperation (median: 7.3 pg/ml (range: 2.5–94.8 pg/ml)). Neutrophil gelatinase-associated lipocalin in plasma peaked 6 h (99.8 ng/ml (52.6–359.1 ng/ml)) and in urine 24 h postoperation (1.6 ng/mg (0.2–6.4 ng/mg)). Urinary kidney injury molecule-1 concentration peaked 24 h postoperation (0.5 ng/mg (0.2–1.2 ng/mg). In vitro, the expression of E-selectin was induced by 20 pg/ml TNF-α. In addition, the expression of interleukin-8, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 was induced by 100 pg/ml TNF-α. Compared to healthy control plasma exposure, postoperative plasma did not increase the expression of markers of endothelial inflammation and adhesion under shear stress in vitro. CONCLUSION: Patients undergoing CPB surgery showed mild systemic inflammation and kidney injury. However, the plasma components did not stimulate endothelial inflammation and adhesion molecule expression in vitro. |
format | Online Article Text |
id | pubmed-8547679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-85476792021-11-09 Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro Ellermann, Sophie F. L. Scheeren, Thomas W. Jongman, Rianne M. Nordhoff, Katja Schnabel, Christiane L. Molema, Grietje Theilmeier, Gregor Meurs, Matijs Van Int J Crit Illn Inj Sci Original Article BACKGROUND: Cardiac surgery with cardiopulmonary bypass (CPB) is commonly associated with acute kidney injury, and microvascular endothelial inflammation is a potential underlying mechanism. We hypothesized that pro-inflammatory components of plasma from patients who underwent coronary artery bypass graft surgery with CPB induce endothelial adhesion molecule expression when incorporating altered shear stress in the in vitro model. METHODS: The clinical characteristics and markers of systemic inflammation and kidney injury were analyzed pre and postoperatively in 29 patients undergoing coronary artery bypass grafting with CPB. The effects of tumor necrosis factor (TNF)-α and patient plasma on the expression of endothelial inflammation and adhesion markers were analyzed in vitro. RESULTS: Plasma TNF-α was elevated 6 h postoperation (median: 7.3 pg/ml (range: 2.5–94.8 pg/ml)). Neutrophil gelatinase-associated lipocalin in plasma peaked 6 h (99.8 ng/ml (52.6–359.1 ng/ml)) and in urine 24 h postoperation (1.6 ng/mg (0.2–6.4 ng/mg)). Urinary kidney injury molecule-1 concentration peaked 24 h postoperation (0.5 ng/mg (0.2–1.2 ng/mg). In vitro, the expression of E-selectin was induced by 20 pg/ml TNF-α. In addition, the expression of interleukin-8, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 was induced by 100 pg/ml TNF-α. Compared to healthy control plasma exposure, postoperative plasma did not increase the expression of markers of endothelial inflammation and adhesion under shear stress in vitro. CONCLUSION: Patients undergoing CPB surgery showed mild systemic inflammation and kidney injury. However, the plasma components did not stimulate endothelial inflammation and adhesion molecule expression in vitro. Wolters Kluwer - Medknow 2021 2021-09-25 /pmc/articles/PMC8547679/ /pubmed/34760660 http://dx.doi.org/10.4103/IJCIIS.IJCIIS_197_20 Text en Copyright: © 2021 International Journal of Critical Illness and Injury Science https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Ellermann, Sophie F. L. Scheeren, Thomas W. Jongman, Rianne M. Nordhoff, Katja Schnabel, Christiane L. Molema, Grietje Theilmeier, Gregor Meurs, Matijs Van Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title | Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title_full | Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title_fullStr | Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title_full_unstemmed | Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title_short | Plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
title_sort | plasma from patients undergoing coronary artery bypass graft surgery does not activate endothelial cells under shear stress in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547679/ https://www.ncbi.nlm.nih.gov/pubmed/34760660 http://dx.doi.org/10.4103/IJCIIS.IJCIIS_197_20 |
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