Single-domain antibody screening by isPLA-seq

Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-through...

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Autores principales: Yin, Yueyuan, Yan, Fei, Zhou, Ruimin, Li, Mingchen, Ma, Jinyi, Liu, Zhe, Ma, Zhenyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548206/
https://www.ncbi.nlm.nih.gov/pubmed/34675071
http://dx.doi.org/10.26508/lsa.202101115
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author Yin, Yueyuan
Yan, Fei
Zhou, Ruimin
Li, Mingchen
Ma, Jinyi
Liu, Zhe
Ma, Zhenyi
author_facet Yin, Yueyuan
Yan, Fei
Zhou, Ruimin
Li, Mingchen
Ma, Jinyi
Liu, Zhe
Ma, Zhenyi
author_sort Yin, Yueyuan
collection PubMed
description Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes.
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spelling pubmed-85482062021-11-05 Single-domain antibody screening by isPLA-seq Yin, Yueyuan Yan, Fei Zhou, Ruimin Li, Mingchen Ma, Jinyi Liu, Zhe Ma, Zhenyi Life Sci Alliance Research Articles Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes. Life Science Alliance LLC 2021-10-21 /pmc/articles/PMC8548206/ /pubmed/34675071 http://dx.doi.org/10.26508/lsa.202101115 Text en © 2021 Yin et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Yin, Yueyuan
Yan, Fei
Zhou, Ruimin
Li, Mingchen
Ma, Jinyi
Liu, Zhe
Ma, Zhenyi
Single-domain antibody screening by isPLA-seq
title Single-domain antibody screening by isPLA-seq
title_full Single-domain antibody screening by isPLA-seq
title_fullStr Single-domain antibody screening by isPLA-seq
title_full_unstemmed Single-domain antibody screening by isPLA-seq
title_short Single-domain antibody screening by isPLA-seq
title_sort single-domain antibody screening by ispla-seq
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548206/
https://www.ncbi.nlm.nih.gov/pubmed/34675071
http://dx.doi.org/10.26508/lsa.202101115
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