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Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection

BACKGROUND: Extraintestinal pathogenic Escherichia coli (ExPEC) causes various infections, including urinary tract infection (UTI), sepsis, and neonatal meningitis. ExPEC strains have virulence factors (VFs) that facilitate infection by allowing bacterial cells to migrate into and multiply within th...

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Autores principales: Kim, Bongyoung, Kim, Jin-Hong, Lee, Yangsoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Laboratory Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548248/
https://www.ncbi.nlm.nih.gov/pubmed/34635614
http://dx.doi.org/10.3343/alm.2022.42.2.203
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author Kim, Bongyoung
Kim, Jin-Hong
Lee, Yangsoon
author_facet Kim, Bongyoung
Kim, Jin-Hong
Lee, Yangsoon
author_sort Kim, Bongyoung
collection PubMed
description BACKGROUND: Extraintestinal pathogenic Escherichia coli (ExPEC) causes various infections, including urinary tract infection (UTI), sepsis, and neonatal meningitis. ExPEC strains have virulence factors (VFs) that facilitate infection by allowing bacterial cells to migrate into and multiply within the host. We compared the microbiological characteristics of ExPEC isolates from blood and urine specimens from UTI patients. METHODS: We conducted a single-center, prospective study in an 855-bed tertiary-care hospital in Korea. We consecutively recruited 80 hospitalized UTI patients with E. coli isolates, which were isolated from blood and/or urine, and urine alone between March 2019 and May 2020. We evaluated the 80 E. coli isolates for the presence of bacterial genes encoding the sequence types (STs), antimicrobial resistance, and VFs using whole-genome sequencing (WGS). RESULTS: We found no significant differences in STs, antimicrobial resistance patterns, or VFs between isolates from blood and urine specimens. ST131, a pandemic multidrug-resistant clone present in both blood and urine, was the most frequent ST (N=19/80, 24%), and ST131 isolates carried more virulence genes, especially, tsh and espC, than non-ST131 isolates. The virulence scores of the ST131 group and the ST69, ST95, and ST1193 groups differed significantly (P<0.05). CONCLUSIONS: We found no STs and VFs associated with bacteremia in WGS data of E. coli isolates from UTI patients. ST131 was the most frequent ST among UTI causing isolates and carried more VF genes than non-ST131 isolates.
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spelling pubmed-85482482022-03-01 Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection Kim, Bongyoung Kim, Jin-Hong Lee, Yangsoon Ann Lab Med Original Article BACKGROUND: Extraintestinal pathogenic Escherichia coli (ExPEC) causes various infections, including urinary tract infection (UTI), sepsis, and neonatal meningitis. ExPEC strains have virulence factors (VFs) that facilitate infection by allowing bacterial cells to migrate into and multiply within the host. We compared the microbiological characteristics of ExPEC isolates from blood and urine specimens from UTI patients. METHODS: We conducted a single-center, prospective study in an 855-bed tertiary-care hospital in Korea. We consecutively recruited 80 hospitalized UTI patients with E. coli isolates, which were isolated from blood and/or urine, and urine alone between March 2019 and May 2020. We evaluated the 80 E. coli isolates for the presence of bacterial genes encoding the sequence types (STs), antimicrobial resistance, and VFs using whole-genome sequencing (WGS). RESULTS: We found no significant differences in STs, antimicrobial resistance patterns, or VFs between isolates from blood and urine specimens. ST131, a pandemic multidrug-resistant clone present in both blood and urine, was the most frequent ST (N=19/80, 24%), and ST131 isolates carried more virulence genes, especially, tsh and espC, than non-ST131 isolates. The virulence scores of the ST131 group and the ST69, ST95, and ST1193 groups differed significantly (P<0.05). CONCLUSIONS: We found no STs and VFs associated with bacteremia in WGS data of E. coli isolates from UTI patients. ST131 was the most frequent ST among UTI causing isolates and carried more VF genes than non-ST131 isolates. Korean Society for Laboratory Medicine 2022-03-01 2022-03-01 /pmc/articles/PMC8548248/ /pubmed/34635614 http://dx.doi.org/10.3343/alm.2022.42.2.203 Text en © Korean Society for Laboratory Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Bongyoung
Kim, Jin-Hong
Lee, Yangsoon
Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title_full Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title_fullStr Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title_full_unstemmed Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title_short Virulence Factors Associated With Escherichia coli Bacteremia and Urinary Tract Infection
title_sort virulence factors associated with escherichia coli bacteremia and urinary tract infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548248/
https://www.ncbi.nlm.nih.gov/pubmed/34635614
http://dx.doi.org/10.3343/alm.2022.42.2.203
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