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Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration

Mouse digit amputation provides a useful model of bone growth after injury, in that the injury promotes intramembranous bone formation in an adult animal. The digit tip is composed of skin, nerves, blood vessels, bones, and tendons, all of which regenerate after digit tip amputation, making it a pow...

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Autores principales: Hoffseth, Kevin, Busse, Emily, Jaramillo, Josue, Simkin, Jennifer, Lacey, Michelle, Sammarco, Mimi C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548682/
https://www.ncbi.nlm.nih.gov/pubmed/34722531
http://dx.doi.org/10.3389/fcell.2021.749055
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author Hoffseth, Kevin
Busse, Emily
Jaramillo, Josue
Simkin, Jennifer
Lacey, Michelle
Sammarco, Mimi C.
author_facet Hoffseth, Kevin
Busse, Emily
Jaramillo, Josue
Simkin, Jennifer
Lacey, Michelle
Sammarco, Mimi C.
author_sort Hoffseth, Kevin
collection PubMed
description Mouse digit amputation provides a useful model of bone growth after injury, in that the injury promotes intramembranous bone formation in an adult animal. The digit tip is composed of skin, nerves, blood vessels, bones, and tendons, all of which regenerate after digit tip amputation, making it a powerful model for multi-tissue regeneration. Bone integrity relies upon a balanced remodeling between bone resorption and formation, which, when disrupted, results in changes to bone architecture and biomechanics, particularly during aging. In this study, we used recently developed techniques to evaluate bone patterning differences between young and aged regenerated bone. This analysis suggests that aged mice have altered trabecular spacing and patterning and increased mineral density of the regenerated bone. To further characterize the biomechanics of regenerated bone, we measured elasticity using a micro-computed tomography image-processing method combined with nanoindentation. This analysis suggests that the regenerated bone demonstrates decreased elasticity compared with the uninjured bone, but there is no significant difference in elasticity between aged and young regenerated bone. These data highlight distinct architectural and biomechanical differences in regenerated bone in both young and aged mice and provide a new analysis tool for the digit amputation model to aid in evaluating the outcomes for potential therapeutic treatments to promote regeneration.
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spelling pubmed-85486822021-10-28 Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration Hoffseth, Kevin Busse, Emily Jaramillo, Josue Simkin, Jennifer Lacey, Michelle Sammarco, Mimi C. Front Cell Dev Biol Cell and Developmental Biology Mouse digit amputation provides a useful model of bone growth after injury, in that the injury promotes intramembranous bone formation in an adult animal. The digit tip is composed of skin, nerves, blood vessels, bones, and tendons, all of which regenerate after digit tip amputation, making it a powerful model for multi-tissue regeneration. Bone integrity relies upon a balanced remodeling between bone resorption and formation, which, when disrupted, results in changes to bone architecture and biomechanics, particularly during aging. In this study, we used recently developed techniques to evaluate bone patterning differences between young and aged regenerated bone. This analysis suggests that aged mice have altered trabecular spacing and patterning and increased mineral density of the regenerated bone. To further characterize the biomechanics of regenerated bone, we measured elasticity using a micro-computed tomography image-processing method combined with nanoindentation. This analysis suggests that the regenerated bone demonstrates decreased elasticity compared with the uninjured bone, but there is no significant difference in elasticity between aged and young regenerated bone. These data highlight distinct architectural and biomechanical differences in regenerated bone in both young and aged mice and provide a new analysis tool for the digit amputation model to aid in evaluating the outcomes for potential therapeutic treatments to promote regeneration. Frontiers Media S.A. 2021-10-13 /pmc/articles/PMC8548682/ /pubmed/34722531 http://dx.doi.org/10.3389/fcell.2021.749055 Text en Copyright © 2021 Hoffseth, Busse, Jaramillo, Simkin, Lacey and Sammarco. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Hoffseth, Kevin
Busse, Emily
Jaramillo, Josue
Simkin, Jennifer
Lacey, Michelle
Sammarco, Mimi C.
Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title_full Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title_fullStr Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title_full_unstemmed Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title_short Age-Dependent Changes in Bone Architecture, Patterning, and Biomechanics During Skeletal Regeneration
title_sort age-dependent changes in bone architecture, patterning, and biomechanics during skeletal regeneration
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548682/
https://www.ncbi.nlm.nih.gov/pubmed/34722531
http://dx.doi.org/10.3389/fcell.2021.749055
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