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Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota
Both fecal microbiota transplantation (FMT) and dietary fiber intervention were verified as effective ways to manipulate the gut microbiota, whereas little is known about the influence of the combined methods on gut microbiota. Here, we constructed “non-industrialized” and “industrialized” gut micro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548821/ https://www.ncbi.nlm.nih.gov/pubmed/34721365 http://dx.doi.org/10.3389/fmicb.2021.757372 |
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author | Zhong, Yifan Cao, Jiahong Deng, Zhaoxi Ma, Yanfei Liu, Jianxin Wang, Haifeng |
author_facet | Zhong, Yifan Cao, Jiahong Deng, Zhaoxi Ma, Yanfei Liu, Jianxin Wang, Haifeng |
author_sort | Zhong, Yifan |
collection | PubMed |
description | Both fecal microbiota transplantation (FMT) and dietary fiber intervention were verified as effective ways to manipulate the gut microbiota, whereas little is known about the influence of the combined methods on gut microbiota. Here, we constructed “non-industrialized” and “industrialized” gut microbiota models to investigate the donor effect of FMT and diet effect in shaping the gut microbiota. Mice were transplanted fecal microbiota from domestic pig and received a diet with low-fiber (D) or high-fiber (DF), whereas the other two groups were transplanted fecal microbiota from wild pig and then received a diet with low-fiber (W) or high-fiber (WF), respectively. Gut microbiota of WF mice showed a lower Shannon and Simpson index (P < 0.05), whereas gut microbiota of W mice showed no significant difference than that of D and DF mice. Random forest models revealed the major differential bacteria genera between four groups, including Anaeroplasma or unclassified_o_Desulfovibrionales, which were influenced by FMT or diet intervention, respectively. Besides, we found a lower out-of-bag rate in the random forest model constructed for dietary fiber (0.086) than that for FMT (0.114). Linear discriminant analysis effective size demonstrated that FMT combined with dietary fiber altered specific gut microbiota, including Alistipes, Clostridium XIVa, Clostridium XI, and Akkermansia, in D, DF, W, and WF mice, respectively. Our results revealed that FMT from different donors coupled with dietary fiber intervention could lead to different patterns of gut microbiota composition, and dietary fiber might play a more critical role in shaping gut microbiota than FMT donor. Strategies based on dietary fiber can influence the effectiveness of FMT in the recipient. |
format | Online Article Text |
id | pubmed-8548821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85488212021-10-28 Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota Zhong, Yifan Cao, Jiahong Deng, Zhaoxi Ma, Yanfei Liu, Jianxin Wang, Haifeng Front Microbiol Microbiology Both fecal microbiota transplantation (FMT) and dietary fiber intervention were verified as effective ways to manipulate the gut microbiota, whereas little is known about the influence of the combined methods on gut microbiota. Here, we constructed “non-industrialized” and “industrialized” gut microbiota models to investigate the donor effect of FMT and diet effect in shaping the gut microbiota. Mice were transplanted fecal microbiota from domestic pig and received a diet with low-fiber (D) or high-fiber (DF), whereas the other two groups were transplanted fecal microbiota from wild pig and then received a diet with low-fiber (W) or high-fiber (WF), respectively. Gut microbiota of WF mice showed a lower Shannon and Simpson index (P < 0.05), whereas gut microbiota of W mice showed no significant difference than that of D and DF mice. Random forest models revealed the major differential bacteria genera between four groups, including Anaeroplasma or unclassified_o_Desulfovibrionales, which were influenced by FMT or diet intervention, respectively. Besides, we found a lower out-of-bag rate in the random forest model constructed for dietary fiber (0.086) than that for FMT (0.114). Linear discriminant analysis effective size demonstrated that FMT combined with dietary fiber altered specific gut microbiota, including Alistipes, Clostridium XIVa, Clostridium XI, and Akkermansia, in D, DF, W, and WF mice, respectively. Our results revealed that FMT from different donors coupled with dietary fiber intervention could lead to different patterns of gut microbiota composition, and dietary fiber might play a more critical role in shaping gut microbiota than FMT donor. Strategies based on dietary fiber can influence the effectiveness of FMT in the recipient. Frontiers Media S.A. 2021-10-13 /pmc/articles/PMC8548821/ /pubmed/34721365 http://dx.doi.org/10.3389/fmicb.2021.757372 Text en Copyright © 2021 Zhong, Cao, Deng, Ma, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhong, Yifan Cao, Jiahong Deng, Zhaoxi Ma, Yanfei Liu, Jianxin Wang, Haifeng Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title | Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title_full | Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title_fullStr | Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title_full_unstemmed | Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title_short | Effect of Fiber and Fecal Microbiota Transplantation Donor on Recipient Mice Gut Microbiota |
title_sort | effect of fiber and fecal microbiota transplantation donor on recipient mice gut microbiota |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548821/ https://www.ncbi.nlm.nih.gov/pubmed/34721365 http://dx.doi.org/10.3389/fmicb.2021.757372 |
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