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The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress

Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex...

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Autores principales: Witzel, Katja, Matros, Andrea, Bertsch, Uwe, Aftab, Tariq, Rutten, Twan, Ramireddy, Eswarayya, Melzer, Michael, Kunze, Gotthard, Mock, Hans-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549704/
https://www.ncbi.nlm.nih.gov/pubmed/34638593
http://dx.doi.org/10.3390/ijms221910248
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author Witzel, Katja
Matros, Andrea
Bertsch, Uwe
Aftab, Tariq
Rutten, Twan
Ramireddy, Eswarayya
Melzer, Michael
Kunze, Gotthard
Mock, Hans-Peter
author_facet Witzel, Katja
Matros, Andrea
Bertsch, Uwe
Aftab, Tariq
Rutten, Twan
Ramireddy, Eswarayya
Melzer, Michael
Kunze, Gotthard
Mock, Hans-Peter
author_sort Witzel, Katja
collection PubMed
description Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex for the transfection of a salt-stress-sensitive yeast strain (Saccharomyces cerevisiae YSH818 Δhog1 mutant). From the retrieved cDNA sequences conferring salt tolerance to the yeast mutant, eleven contained the coding sequence of a jacalin-related lectin (JRL) that shows homology to the previously identified JRL horcolin from barley coleoptiles that we therefore named the gene HvHorcH. The detection of HvHorcH protein in root extracellular fluid suggests a secretion under stress conditions. Furthermore, HvHorcH exhibited specificity towards mannose. Protein abundance of HvHorcH in roots of salt-sensitive or salt-tolerant barley cultivars were not trait-specific to salinity treatment, but protein levels increased in response to the treatment, particularly in the root tip. Expression of HvHorcH in Arabidopsis thaliana root tips increased salt tolerance. Hence, we conclude that this protein is involved in the adaptation of plants to salinity.
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spelling pubmed-85497042021-10-28 The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress Witzel, Katja Matros, Andrea Bertsch, Uwe Aftab, Tariq Rutten, Twan Ramireddy, Eswarayya Melzer, Michael Kunze, Gotthard Mock, Hans-Peter Int J Mol Sci Article Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex for the transfection of a salt-stress-sensitive yeast strain (Saccharomyces cerevisiae YSH818 Δhog1 mutant). From the retrieved cDNA sequences conferring salt tolerance to the yeast mutant, eleven contained the coding sequence of a jacalin-related lectin (JRL) that shows homology to the previously identified JRL horcolin from barley coleoptiles that we therefore named the gene HvHorcH. The detection of HvHorcH protein in root extracellular fluid suggests a secretion under stress conditions. Furthermore, HvHorcH exhibited specificity towards mannose. Protein abundance of HvHorcH in roots of salt-sensitive or salt-tolerant barley cultivars were not trait-specific to salinity treatment, but protein levels increased in response to the treatment, particularly in the root tip. Expression of HvHorcH in Arabidopsis thaliana root tips increased salt tolerance. Hence, we conclude that this protein is involved in the adaptation of plants to salinity. MDPI 2021-09-23 /pmc/articles/PMC8549704/ /pubmed/34638593 http://dx.doi.org/10.3390/ijms221910248 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Witzel, Katja
Matros, Andrea
Bertsch, Uwe
Aftab, Tariq
Rutten, Twan
Ramireddy, Eswarayya
Melzer, Michael
Kunze, Gotthard
Mock, Hans-Peter
The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title_full The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title_fullStr The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title_full_unstemmed The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title_short The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
title_sort jacalin-related lectin hvhorch is involved in the physiological response of barley roots to salt stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549704/
https://www.ncbi.nlm.nih.gov/pubmed/34638593
http://dx.doi.org/10.3390/ijms221910248
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