Cargando…
Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential
While many studies have described Drosophila embryonic and larval blood cells, the hematopoietic system of the imago remains poorly characterized and conflicting data have been published concerning adult hematopoiesis. Using a combination of blood cell markers, we show that the adult hematopoietic s...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550105/ https://www.ncbi.nlm.nih.gov/pubmed/34722521 http://dx.doi.org/10.3389/fcell.2021.739357 |
_version_ | 1784590892090261504 |
---|---|
author | Boulet, Manon Renaud, Yoan Lapraz, François Benmimoun, Billel Vandel, Laurence Waltzer, Lucas |
author_facet | Boulet, Manon Renaud, Yoan Lapraz, François Benmimoun, Billel Vandel, Laurence Waltzer, Lucas |
author_sort | Boulet, Manon |
collection | PubMed |
description | While many studies have described Drosophila embryonic and larval blood cells, the hematopoietic system of the imago remains poorly characterized and conflicting data have been published concerning adult hematopoiesis. Using a combination of blood cell markers, we show that the adult hematopoietic system is essentially composed of a few distinct mature blood cell types. In addition, our transcriptomics results indicate that adult and larval blood cells have both common and specific features and it appears that adult hemocytes reactivate many genes expressed in embryonic blood cells. Interestingly, we identify a small set of blood cells that does not express differentiation markers but rather maintains the expression of the progenitor marker domeMeso. Yet, we show that these cells are derived from the posterior signaling center, a specialized population of cells present in the larval lymph gland, rather than from larval blood cell progenitors, and that their maintenance depends on the EBF transcription factor Collier. Furthermore, while these cells are normally quiescent, we find that some of them can differentiate and proliferate in response to bacterial infection. In sum, our results indicate that adult flies harbor a small population of specialized cells with limited hematopoietic potential and further support the idea that no substantial hematopoiesis takes place during adulthood. |
format | Online Article Text |
id | pubmed-8550105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85501052021-10-28 Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential Boulet, Manon Renaud, Yoan Lapraz, François Benmimoun, Billel Vandel, Laurence Waltzer, Lucas Front Cell Dev Biol Cell and Developmental Biology While many studies have described Drosophila embryonic and larval blood cells, the hematopoietic system of the imago remains poorly characterized and conflicting data have been published concerning adult hematopoiesis. Using a combination of blood cell markers, we show that the adult hematopoietic system is essentially composed of a few distinct mature blood cell types. In addition, our transcriptomics results indicate that adult and larval blood cells have both common and specific features and it appears that adult hemocytes reactivate many genes expressed in embryonic blood cells. Interestingly, we identify a small set of blood cells that does not express differentiation markers but rather maintains the expression of the progenitor marker domeMeso. Yet, we show that these cells are derived from the posterior signaling center, a specialized population of cells present in the larval lymph gland, rather than from larval blood cell progenitors, and that their maintenance depends on the EBF transcription factor Collier. Furthermore, while these cells are normally quiescent, we find that some of them can differentiate and proliferate in response to bacterial infection. In sum, our results indicate that adult flies harbor a small population of specialized cells with limited hematopoietic potential and further support the idea that no substantial hematopoiesis takes place during adulthood. Frontiers Media S.A. 2021-10-13 /pmc/articles/PMC8550105/ /pubmed/34722521 http://dx.doi.org/10.3389/fcell.2021.739357 Text en Copyright © 2021 Boulet, Renaud, Lapraz, Benmimoun, Vandel and Waltzer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Boulet, Manon Renaud, Yoan Lapraz, François Benmimoun, Billel Vandel, Laurence Waltzer, Lucas Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title | Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title_full | Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title_fullStr | Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title_full_unstemmed | Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title_short | Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential |
title_sort | characterization of the drosophila adult hematopoietic system reveals a rare cell population with differentiation and proliferation potential |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550105/ https://www.ncbi.nlm.nih.gov/pubmed/34722521 http://dx.doi.org/10.3389/fcell.2021.739357 |
work_keys_str_mv | AT bouletmanon characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential AT renaudyoan characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential AT laprazfrancois characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential AT benmimounbillel characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential AT vandellaurence characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential AT waltzerlucas characterizationofthedrosophilaadulthematopoieticsystemrevealsararecellpopulationwithdifferentiationandproliferationpotential |