Cargando…
Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance
Oxidative stress (OS) refers to endogenous and/or exogenous stimulation when the balance between oxidation and antioxidants in the body is disrupted, resulting in excessive production of free radicals. Excessive free radicals exert a series of negative effects on the body, which can result in the ox...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550864/ https://www.ncbi.nlm.nih.gov/pubmed/34721758 http://dx.doi.org/10.1155/2021/5561124 |
_version_ | 1784591046019121152 |
---|---|
author | Che, Xiangyu Qi, Xiaochen Xu, Yingkun Wang, Qifei Wu, Guangzhen |
author_facet | Che, Xiangyu Qi, Xiaochen Xu, Yingkun Wang, Qifei Wu, Guangzhen |
author_sort | Che, Xiangyu |
collection | PubMed |
description | Oxidative stress (OS) refers to endogenous and/or exogenous stimulation when the balance between oxidation and antioxidants in the body is disrupted, resulting in excessive production of free radicals. Excessive free radicals exert a series of negative effects on the body, which can result in the oxidation of and infliction of damage on biological molecules and further cause cell death and tissue damage, which are related to many pathological processes. Pathways related to OS have always been the focus of medical research. Several studies are being conducted to develop strategies to treat cancer by exploring the OS pathways. Therefore, this study is aimed at determining the correlation between the OS pathway and kidney renal clear cell carcinoma (KIRC) through bioinformatics analysis, at proving the effect of common anticancer drugs on the OS pathway, and at constructing a prognosis model of patients with KIRC based on several genes with the strongest correlation between the OS pathway and KIRC. We first collected and analyzed gene expression and clinical information of related patients through TCGA database. Then, we divided the samples into three clusters according to their gene expression levels obtained through cluster analysis. Using these three clusters, we performed GDSC drug analysis and GSEA analysis and examined the correlation among the OS pathway, histone modification, and immune cell infiltration. We also analyzed the response of anti-PD-1 and anti-CTLA-4 to the OS pathway. Thereafter, we used LASSO regression to select the most suitable nine genes, combined with the clinicopathological characteristics to establish the prognosis model of patients with KIRC, and verified the scientific precision of the model. Finally, tumor mutational burden was calculated to verify whether patients would benefit from immunotherapy. The results of this study may provide a reference for the establishment of treatment strategies for patients with KIRC. |
format | Online Article Text |
id | pubmed-8550864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-85508642021-10-28 Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance Che, Xiangyu Qi, Xiaochen Xu, Yingkun Wang, Qifei Wu, Guangzhen Oxid Med Cell Longev Research Article Oxidative stress (OS) refers to endogenous and/or exogenous stimulation when the balance between oxidation and antioxidants in the body is disrupted, resulting in excessive production of free radicals. Excessive free radicals exert a series of negative effects on the body, which can result in the oxidation of and infliction of damage on biological molecules and further cause cell death and tissue damage, which are related to many pathological processes. Pathways related to OS have always been the focus of medical research. Several studies are being conducted to develop strategies to treat cancer by exploring the OS pathways. Therefore, this study is aimed at determining the correlation between the OS pathway and kidney renal clear cell carcinoma (KIRC) through bioinformatics analysis, at proving the effect of common anticancer drugs on the OS pathway, and at constructing a prognosis model of patients with KIRC based on several genes with the strongest correlation between the OS pathway and KIRC. We first collected and analyzed gene expression and clinical information of related patients through TCGA database. Then, we divided the samples into three clusters according to their gene expression levels obtained through cluster analysis. Using these three clusters, we performed GDSC drug analysis and GSEA analysis and examined the correlation among the OS pathway, histone modification, and immune cell infiltration. We also analyzed the response of anti-PD-1 and anti-CTLA-4 to the OS pathway. Thereafter, we used LASSO regression to select the most suitable nine genes, combined with the clinicopathological characteristics to establish the prognosis model of patients with KIRC, and verified the scientific precision of the model. Finally, tumor mutational burden was calculated to verify whether patients would benefit from immunotherapy. The results of this study may provide a reference for the establishment of treatment strategies for patients with KIRC. Hindawi 2021-10-20 /pmc/articles/PMC8550864/ /pubmed/34721758 http://dx.doi.org/10.1155/2021/5561124 Text en Copyright © 2021 Xiangyu Che et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Che, Xiangyu Qi, Xiaochen Xu, Yingkun Wang, Qifei Wu, Guangzhen Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title | Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title_full | Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title_fullStr | Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title_full_unstemmed | Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title_short | Using Genomic and Transcriptome Analyses to Identify the Role of the Oxidative Stress Pathway in Renal Clear Cell Carcinoma and Its Potential Therapeutic Significance |
title_sort | using genomic and transcriptome analyses to identify the role of the oxidative stress pathway in renal clear cell carcinoma and its potential therapeutic significance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550864/ https://www.ncbi.nlm.nih.gov/pubmed/34721758 http://dx.doi.org/10.1155/2021/5561124 |
work_keys_str_mv | AT chexiangyu usinggenomicandtranscriptomeanalysestoidentifytheroleoftheoxidativestresspathwayinrenalclearcellcarcinomaanditspotentialtherapeuticsignificance AT qixiaochen usinggenomicandtranscriptomeanalysestoidentifytheroleoftheoxidativestresspathwayinrenalclearcellcarcinomaanditspotentialtherapeuticsignificance AT xuyingkun usinggenomicandtranscriptomeanalysestoidentifytheroleoftheoxidativestresspathwayinrenalclearcellcarcinomaanditspotentialtherapeuticsignificance AT wangqifei usinggenomicandtranscriptomeanalysestoidentifytheroleoftheoxidativestresspathwayinrenalclearcellcarcinomaanditspotentialtherapeuticsignificance AT wuguangzhen usinggenomicandtranscriptomeanalysestoidentifytheroleoftheoxidativestresspathwayinrenalclearcellcarcinomaanditspotentialtherapeuticsignificance |