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SARS-CoV-2 infection negatively affects ovarian function in ART patients

Several organs, such as the heart, breasts, intestine, testes, and ovaries, have been reported to be target tissues of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To date, no studies have demonstrated SARS-CoV-2 infection in the female reproductive system. In the pres...

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Autores principales: Herrero, Yamila, Pascuali, Natalia, Velázquez, Candela, Oubiña, Gonzalo, Hauk, Vanesa, de Zúñiga, Ignacio, Peña, Mariana Gómez, Martínez, Gustavo, Lavolpe, Mariano, Veiga, Florencia, Neuspiller, Fernando, Abramovich, Dalhia, Scotti, Leopoldina, Parborell, Fernanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550892/
https://www.ncbi.nlm.nih.gov/pubmed/34718118
http://dx.doi.org/10.1016/j.bbadis.2021.166295
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author Herrero, Yamila
Pascuali, Natalia
Velázquez, Candela
Oubiña, Gonzalo
Hauk, Vanesa
de Zúñiga, Ignacio
Peña, Mariana Gómez
Martínez, Gustavo
Lavolpe, Mariano
Veiga, Florencia
Neuspiller, Fernando
Abramovich, Dalhia
Scotti, Leopoldina
Parborell, Fernanda
author_facet Herrero, Yamila
Pascuali, Natalia
Velázquez, Candela
Oubiña, Gonzalo
Hauk, Vanesa
de Zúñiga, Ignacio
Peña, Mariana Gómez
Martínez, Gustavo
Lavolpe, Mariano
Veiga, Florencia
Neuspiller, Fernando
Abramovich, Dalhia
Scotti, Leopoldina
Parborell, Fernanda
author_sort Herrero, Yamila
collection PubMed
description Several organs, such as the heart, breasts, intestine, testes, and ovaries, have been reported to be target tissues of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To date, no studies have demonstrated SARS-CoV-2 infection in the female reproductive system. In the present study, we investigated the effects of SARS-CoV-2 infection on ovarian function by comparing follicular fluid (FF) from control and recovered coronavirus disease 2019 (COVID-19) patients and by evaluating the influence of these FF on human endothelial and non-luteinized granulosa cell cultures. Our results showed that most FFs (91.3%) from screened post COVID-19 patients were positive for IgG antibodies against SARS-CoV-2. Additionally, patients with higher levels of IgG against SARS-CoV-2 had lower numbers of retrieved oocytes. While VEGF and IL-1β were significantly lower in post COVID-19 FF, IL-10 did not differ from that in control FF. Moreover, in COV434 cells stimulated with FF from post COVID-19 patients, steroidogenic acute regulatory protein (StAR), estrogen-receptor β (Erβ), and vascular endothelial growth factor (VEGF) expression were significantly decreased, whereas estrogen-receptor α (ERα) and 3β-hydroxysteroid dehydrogenase (3β-HSD) did not change. In endothelial cells stimulated with post COVID-19 FF, we observed a decrease in cell migration without changes in protein expression of certain angiogenic factors. Both cell types showed a significantly higher γH2AX expression when exposed to post COVID-19 FF. In conclusion, our results describe for the first time that the SARS-CoV-2 infection adversely affects the follicular microenvironment, thus dysregulating ovarian function.
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spelling pubmed-85508922021-10-28 SARS-CoV-2 infection negatively affects ovarian function in ART patients Herrero, Yamila Pascuali, Natalia Velázquez, Candela Oubiña, Gonzalo Hauk, Vanesa de Zúñiga, Ignacio Peña, Mariana Gómez Martínez, Gustavo Lavolpe, Mariano Veiga, Florencia Neuspiller, Fernando Abramovich, Dalhia Scotti, Leopoldina Parborell, Fernanda Biochim Biophys Acta Mol Basis Dis Article Several organs, such as the heart, breasts, intestine, testes, and ovaries, have been reported to be target tissues of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To date, no studies have demonstrated SARS-CoV-2 infection in the female reproductive system. In the present study, we investigated the effects of SARS-CoV-2 infection on ovarian function by comparing follicular fluid (FF) from control and recovered coronavirus disease 2019 (COVID-19) patients and by evaluating the influence of these FF on human endothelial and non-luteinized granulosa cell cultures. Our results showed that most FFs (91.3%) from screened post COVID-19 patients were positive for IgG antibodies against SARS-CoV-2. Additionally, patients with higher levels of IgG against SARS-CoV-2 had lower numbers of retrieved oocytes. While VEGF and IL-1β were significantly lower in post COVID-19 FF, IL-10 did not differ from that in control FF. Moreover, in COV434 cells stimulated with FF from post COVID-19 patients, steroidogenic acute regulatory protein (StAR), estrogen-receptor β (Erβ), and vascular endothelial growth factor (VEGF) expression were significantly decreased, whereas estrogen-receptor α (ERα) and 3β-hydroxysteroid dehydrogenase (3β-HSD) did not change. In endothelial cells stimulated with post COVID-19 FF, we observed a decrease in cell migration without changes in protein expression of certain angiogenic factors. Both cell types showed a significantly higher γH2AX expression when exposed to post COVID-19 FF. In conclusion, our results describe for the first time that the SARS-CoV-2 infection adversely affects the follicular microenvironment, thus dysregulating ovarian function. Elsevier B.V. 2022-01-01 2021-10-27 /pmc/articles/PMC8550892/ /pubmed/34718118 http://dx.doi.org/10.1016/j.bbadis.2021.166295 Text en © 2021 Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Herrero, Yamila
Pascuali, Natalia
Velázquez, Candela
Oubiña, Gonzalo
Hauk, Vanesa
de Zúñiga, Ignacio
Peña, Mariana Gómez
Martínez, Gustavo
Lavolpe, Mariano
Veiga, Florencia
Neuspiller, Fernando
Abramovich, Dalhia
Scotti, Leopoldina
Parborell, Fernanda
SARS-CoV-2 infection negatively affects ovarian function in ART patients
title SARS-CoV-2 infection negatively affects ovarian function in ART patients
title_full SARS-CoV-2 infection negatively affects ovarian function in ART patients
title_fullStr SARS-CoV-2 infection negatively affects ovarian function in ART patients
title_full_unstemmed SARS-CoV-2 infection negatively affects ovarian function in ART patients
title_short SARS-CoV-2 infection negatively affects ovarian function in ART patients
title_sort sars-cov-2 infection negatively affects ovarian function in art patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550892/
https://www.ncbi.nlm.nih.gov/pubmed/34718118
http://dx.doi.org/10.1016/j.bbadis.2021.166295
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