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The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities
Plant-associated microorganisms are known to contribute with various beneficial functions to the health and productivity of their hosts, yet the microbiome of most plants remains unexplored. This especially applies to wild relatives of cultivated plants, which might harbor beneficial microorganisms...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551121/ https://www.ncbi.nlm.nih.gov/pubmed/33723621 http://dx.doi.org/10.1007/s00248-021-01728-5 |
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author | Chen, Xiaoyulong Krug, Lisa Yang, Maofa Berg, Gabriele Cernava, Tomislav |
author_facet | Chen, Xiaoyulong Krug, Lisa Yang, Maofa Berg, Gabriele Cernava, Tomislav |
author_sort | Chen, Xiaoyulong |
collection | PubMed |
description | Plant-associated microorganisms are known to contribute with various beneficial functions to the health and productivity of their hosts, yet the microbiome of most plants remains unexplored. This especially applies to wild relatives of cultivated plants, which might harbor beneficial microorganisms that were lost during intensive breeding. We studied bacterial communities of the Himalayan onion (Allium wallichii Kunth), a wild relative of onion native to mountains in East Asia. The bacterial community structure was assessed in different plant microhabitats (rhizosphere, endosphere, anthosphere) by sequencing of 16S rRNA gene fragment amplicons. Targeted bioinformatic analyses were implemented in order to identify unique features in each habitat and to map the overall community in the first representative of the Amaryllidaceae plant family. The highest bacterial diversity was found for bulk soil (Shannon index, H′ 9.3) at the high-altitude sampling location. It was followed by the plant rhizosphere (H′ 8.9) while communities colonizing flowers (H′ 6.1) and the endosphere (H′ 6.5 and 5.6) where less diverse. Interestingly, we observed a non-significant rhizosphere effect. Another specificity of the microbiome was its high evenness in taxonomic distribution, which was so far not observed in plant microbiomes. Pseudomonas was identified among additional 10 bacterial genera as a plant-specific signature. The first insights into the microbiome of a plant in the widespread Allium genus will facilitate upcoming comparisons with its domesticated relatives while additionally providing a detailed microbiome mapping of the plant’s microhabitats to facilitate bioresource mining. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-021-01728-5. |
format | Online Article Text |
id | pubmed-8551121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-85511212021-10-29 The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities Chen, Xiaoyulong Krug, Lisa Yang, Maofa Berg, Gabriele Cernava, Tomislav Microb Ecol Plant Microbe Interactions Plant-associated microorganisms are known to contribute with various beneficial functions to the health and productivity of their hosts, yet the microbiome of most plants remains unexplored. This especially applies to wild relatives of cultivated plants, which might harbor beneficial microorganisms that were lost during intensive breeding. We studied bacterial communities of the Himalayan onion (Allium wallichii Kunth), a wild relative of onion native to mountains in East Asia. The bacterial community structure was assessed in different plant microhabitats (rhizosphere, endosphere, anthosphere) by sequencing of 16S rRNA gene fragment amplicons. Targeted bioinformatic analyses were implemented in order to identify unique features in each habitat and to map the overall community in the first representative of the Amaryllidaceae plant family. The highest bacterial diversity was found for bulk soil (Shannon index, H′ 9.3) at the high-altitude sampling location. It was followed by the plant rhizosphere (H′ 8.9) while communities colonizing flowers (H′ 6.1) and the endosphere (H′ 6.5 and 5.6) where less diverse. Interestingly, we observed a non-significant rhizosphere effect. Another specificity of the microbiome was its high evenness in taxonomic distribution, which was so far not observed in plant microbiomes. Pseudomonas was identified among additional 10 bacterial genera as a plant-specific signature. The first insights into the microbiome of a plant in the widespread Allium genus will facilitate upcoming comparisons with its domesticated relatives while additionally providing a detailed microbiome mapping of the plant’s microhabitats to facilitate bioresource mining. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-021-01728-5. Springer US 2021-03-16 2021 /pmc/articles/PMC8551121/ /pubmed/33723621 http://dx.doi.org/10.1007/s00248-021-01728-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Plant Microbe Interactions Chen, Xiaoyulong Krug, Lisa Yang, Maofa Berg, Gabriele Cernava, Tomislav The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title | The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title_full | The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title_fullStr | The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title_full_unstemmed | The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title_short | The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities |
title_sort | himalayan onion (allium wallichii kunth) harbors unique spatially organized bacterial communities |
topic | Plant Microbe Interactions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551121/ https://www.ncbi.nlm.nih.gov/pubmed/33723621 http://dx.doi.org/10.1007/s00248-021-01728-5 |
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