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Dissociation of human oral mucosal tissue for single-cell applications

Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of heterogene...

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Detalles Bibliográficos
Autores principales: Greenwell-Wild, Teresa, Williams, Drake Winslow, Moutsopoulos, Niki Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551218/
https://www.ncbi.nlm.nih.gov/pubmed/34746866
http://dx.doi.org/10.1016/j.xpro.2021.100908