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Dissociation of human oral mucosal tissue for single-cell applications
Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of heterogene...
Autores principales: | Greenwell-Wild, Teresa, Williams, Drake Winslow, Moutsopoulos, Niki Maria |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551218/ https://www.ncbi.nlm.nih.gov/pubmed/34746866 http://dx.doi.org/10.1016/j.xpro.2021.100908 |
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