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Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials
Intensive research has been conducted with the aim of developing dental restorative/prosthetic materials with antibacterial and anti-biofilm effects that contribute to controlling bacterial infection in the oral cavity. In situ evaluations were performed to assess the clinical efficacy of these mate...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551238/ https://www.ncbi.nlm.nih.gov/pubmed/34707212 http://dx.doi.org/10.1038/s41598-021-00803-8 |
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author | Kohno, Tomoki Kitagawa, Haruaki Tsuboi, Ririko Nishimura, Yuma Imazato, Satoshi |
author_facet | Kohno, Tomoki Kitagawa, Haruaki Tsuboi, Ririko Nishimura, Yuma Imazato, Satoshi |
author_sort | Kohno, Tomoki |
collection | PubMed |
description | Intensive research has been conducted with the aim of developing dental restorative/prosthetic materials with antibacterial and anti-biofilm effects that contribute to controlling bacterial infection in the oral cavity. In situ evaluations were performed to assess the clinical efficacy of these materials by exposing them to oral environments. However, it is difficult to recruit many participants to collect sufficient amount of data for scientific analysis. This study aimed to assemble an original flow-cell type bioreactor equipped with two flow routes and assess its usefulness by evaluating the ability to reproduce in situ oral biofilms formed on restorative materials. A drop of bacterial suspension collected from human saliva and 0.2% sucrose solution was introduced into the assembled bioreactor while maintaining the incubation conditions. The bioreactor was able to mimic the number of bacterial cells, live/dead bacterial volume, and volume fraction of live bacteria in the in situ oral biofilm formed on the surface of restorative materials. The usefulness of the established culture system was further validated by a clear demonstration of the anti-biofilm effects of a glass-ionomer cement incorporating zinc-releasing glasses when evaluated by this system. |
format | Online Article Text |
id | pubmed-8551238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85512382021-10-28 Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials Kohno, Tomoki Kitagawa, Haruaki Tsuboi, Ririko Nishimura, Yuma Imazato, Satoshi Sci Rep Article Intensive research has been conducted with the aim of developing dental restorative/prosthetic materials with antibacterial and anti-biofilm effects that contribute to controlling bacterial infection in the oral cavity. In situ evaluations were performed to assess the clinical efficacy of these materials by exposing them to oral environments. However, it is difficult to recruit many participants to collect sufficient amount of data for scientific analysis. This study aimed to assemble an original flow-cell type bioreactor equipped with two flow routes and assess its usefulness by evaluating the ability to reproduce in situ oral biofilms formed on restorative materials. A drop of bacterial suspension collected from human saliva and 0.2% sucrose solution was introduced into the assembled bioreactor while maintaining the incubation conditions. The bioreactor was able to mimic the number of bacterial cells, live/dead bacterial volume, and volume fraction of live bacteria in the in situ oral biofilm formed on the surface of restorative materials. The usefulness of the established culture system was further validated by a clear demonstration of the anti-biofilm effects of a glass-ionomer cement incorporating zinc-releasing glasses when evaluated by this system. Nature Publishing Group UK 2021-10-27 /pmc/articles/PMC8551238/ /pubmed/34707212 http://dx.doi.org/10.1038/s41598-021-00803-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kohno, Tomoki Kitagawa, Haruaki Tsuboi, Ririko Nishimura, Yuma Imazato, Satoshi Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title | Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title_full | Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title_fullStr | Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title_full_unstemmed | Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title_short | Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
title_sort | establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551238/ https://www.ncbi.nlm.nih.gov/pubmed/34707212 http://dx.doi.org/10.1038/s41598-021-00803-8 |
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