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Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye

Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus...

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Autores principales: Mehta, Abijeet Singh, Deshpande, Prajakta, Chimata, Anuradha Venkatakrishnan, Tsonis, Panagiotis A., Singh, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551474/
https://www.ncbi.nlm.nih.gov/pubmed/34746690
http://dx.doi.org/10.1016/j.isci.2021.103166
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author Mehta, Abijeet Singh
Deshpande, Prajakta
Chimata, Anuradha Venkatakrishnan
Tsonis, Panagiotis A.
Singh, Amit
author_facet Mehta, Abijeet Singh
Deshpande, Prajakta
Chimata, Anuradha Venkatakrishnan
Tsonis, Panagiotis A.
Singh, Amit
author_sort Mehta, Abijeet Singh
collection PubMed
description Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna5), which were ectopically expressed in L(2) mutant and GMR-hid, GMR-GAL4 eye. L(2) exhibits the loss of ventral half of early eye and head involution defective (hid) triggers cell-death during later eye development. Surprisingly, newt genes significantly restore missing photoreceptor cells both in L(2) and GMR>hid background by upregulating cell-proliferation and blocking cell-death, regulating evolutionarily conserved Wingless (Wg)/Wnt signaling pathway and exhibit non-cell-autonomous rescues. Further, Wg/Wnt signaling acts downstream of newt genes. Our data highlights that unique newt proteins can regulate conserved pathways to trigger a robust restoration of missing photoreceptor cells in Drosophila eye model with weak restoration capability.
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spelling pubmed-85514742021-11-04 Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye Mehta, Abijeet Singh Deshpande, Prajakta Chimata, Anuradha Venkatakrishnan Tsonis, Panagiotis A. Singh, Amit iScience Article Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna5), which were ectopically expressed in L(2) mutant and GMR-hid, GMR-GAL4 eye. L(2) exhibits the loss of ventral half of early eye and head involution defective (hid) triggers cell-death during later eye development. Surprisingly, newt genes significantly restore missing photoreceptor cells both in L(2) and GMR>hid background by upregulating cell-proliferation and blocking cell-death, regulating evolutionarily conserved Wingless (Wg)/Wnt signaling pathway and exhibit non-cell-autonomous rescues. Further, Wg/Wnt signaling acts downstream of newt genes. Our data highlights that unique newt proteins can regulate conserved pathways to trigger a robust restoration of missing photoreceptor cells in Drosophila eye model with weak restoration capability. Elsevier 2021-09-24 /pmc/articles/PMC8551474/ /pubmed/34746690 http://dx.doi.org/10.1016/j.isci.2021.103166 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Mehta, Abijeet Singh
Deshpande, Prajakta
Chimata, Anuradha Venkatakrishnan
Tsonis, Panagiotis A.
Singh, Amit
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title_full Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title_fullStr Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title_full_unstemmed Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title_short Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
title_sort newt regeneration genes regulate wingless signaling to restore patterning in drosophila eye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551474/
https://www.ncbi.nlm.nih.gov/pubmed/34746690
http://dx.doi.org/10.1016/j.isci.2021.103166
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