Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca(2+)) handling, and action...

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Detalles Bibliográficos
Autores principales: Zhang, Joe Z., Zhao, Shane Rui, Tu, Chengyi, Pang, Paul, Zhang, Mao, Wu, Joseph C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551496/
https://www.ncbi.nlm.nih.gov/pubmed/34746854
http://dx.doi.org/10.1016/j.xpro.2021.100859
Descripción
Sumario:Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca(2+)) handling, and action potential. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

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