Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca(2+)) handling, and action...

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Detalles Bibliográficos
Autores principales: Zhang, Joe Z., Zhao, Shane Rui, Tu, Chengyi, Pang, Paul, Zhang, Mao, Wu, Joseph C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551496/
https://www.ncbi.nlm.nih.gov/pubmed/34746854
http://dx.doi.org/10.1016/j.xpro.2021.100859
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author Zhang, Joe Z.
Zhao, Shane Rui
Tu, Chengyi
Pang, Paul
Zhang, Mao
Wu, Joseph C.
author_facet Zhang, Joe Z.
Zhao, Shane Rui
Tu, Chengyi
Pang, Paul
Zhang, Mao
Wu, Joseph C.
author_sort Zhang, Joe Z.
collection PubMed
description Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca(2+)) handling, and action potential. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
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spelling pubmed-85514962021-11-04 Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes Zhang, Joe Z. Zhao, Shane Rui Tu, Chengyi Pang, Paul Zhang, Mao Wu, Joseph C. STAR Protoc Protocol Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca(2+)) handling, and action potential. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021). Elsevier 2021-10-08 /pmc/articles/PMC8551496/ /pubmed/34746854 http://dx.doi.org/10.1016/j.xpro.2021.100859 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Joe Z.
Zhao, Shane Rui
Tu, Chengyi
Pang, Paul
Zhang, Mao
Wu, Joseph C.
Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_full Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_fullStr Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_full_unstemmed Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_short Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_sort protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551496/
https://www.ncbi.nlm.nih.gov/pubmed/34746854
http://dx.doi.org/10.1016/j.xpro.2021.100859
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