Purification of full-length recombinant human huntingtin proteins with allelic series of polyglutamine lengths

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by the polyglutamine (polyQ) expansion in huntingtin (HTT) protein. The challenge of obtaining full-length HTT proteins with high purity limits the understanding of the HTT protein function. Here, we provide a...

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Detalles Bibliográficos
Autores principales: Kim, Hyeongju, Hyun, Kyung-gi, Lloret, Alejandro, Seong, Ihn Sik, Song, Ji-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551504/
https://www.ncbi.nlm.nih.gov/pubmed/34746859
http://dx.doi.org/10.1016/j.xpro.2021.100886
Descripción
Sumario:Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by the polyglutamine (polyQ) expansion in huntingtin (HTT) protein. The challenge of obtaining full-length HTT proteins with high purity limits the understanding of the HTT protein function. Here, we provide a protocol to generate and purify full-length recombinant human HTT proteins with various polyQ lengths, which is key to investigate the biochemical function of HTT proteins and the molecular mechanism underlying HD pathology. For complete details on the use and execution of this protocol, please refer to Jung et al. (2020).

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