Cargando…
Association between urinary VEGFA and renal pathology of IgA nephropathy patients
BACKGROUND: Renal biopsy remains the golden standard for diagnosing and monitoring IgA nephropathy (IgAN). Vascular endothelial growth factor A (VEGFA) was crucial for the survival of glomerular cells. Our aim was to screen the expression pattern of urinary, circulating and renal VEGFA in IgAN patie...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551689/ https://www.ncbi.nlm.nih.gov/pubmed/34498313 http://dx.doi.org/10.1002/jcla.23995 |
_version_ | 1784591214773796864 |
---|---|
author | Feng, Shaozhen Huang, Naya Xue, Miaorong Zhang, Puhua Zhong, Zhong Guo, Qunying Li, Zhijian |
author_facet | Feng, Shaozhen Huang, Naya Xue, Miaorong Zhang, Puhua Zhong, Zhong Guo, Qunying Li, Zhijian |
author_sort | Feng, Shaozhen |
collection | PubMed |
description | BACKGROUND: Renal biopsy remains the golden standard for diagnosing and monitoring IgA nephropathy (IgAN). Vascular endothelial growth factor A (VEGFA) was crucial for the survival of glomerular cells. Our aim was to screen the expression pattern of urinary, circulating and renal VEGFA in IgAN patients to reveal their relationship with renal pathology and outcomes. METHODS: Baseline VEGFA levels were determined with ELISA, real‐time PCR and immunohistochemistry. Associations between VEGFA expression and clinical–pathological parameters, and renal outcomes were evaluated. RESULTS: Compared with healthy controls, urinary VEGFA level was obviously elevated in IgAN patients (76.19 ± 63.67 pg/mg Cr vs 146.67 ± 232.71 pg/mg Cr, p = 0.0291) and not correlated with serum VEGFA level. Baseline urinary VEGFA was significantly associated with gender and tubular atrophy/interstitial fibrosis by stepwise multivariate regression analysis. Urinary VEGFA was higher in male patients accompanied with higher serum creatinine, larger proportion of hypertension and recurrent hematuria than in female patients. In the kidney of IgAN patients, VEGFA were robustly expressed in the parietal epithelial cells, podocytes, mesangial cells and tubular epithelial cells. After a follow‐up duration of 38.53 ± 27.14 months, IgAN patients with higher urinary VEGFA level were found to have a poorer renal outcome of renal replacement therapy (HR = 1.027, p = 0.037) or composite outcome (HR = 1.023, p = 0.039) after adjusting for confounders. CONCLUSIONS: Increased urinary VEGFA might reflect certain renal pathology and, although not fully specific, still could be served as a valuable noninvasive indicator in predicting renal progression of IgAN. |
format | Online Article Text |
id | pubmed-8551689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85516892021-11-04 Association between urinary VEGFA and renal pathology of IgA nephropathy patients Feng, Shaozhen Huang, Naya Xue, Miaorong Zhang, Puhua Zhong, Zhong Guo, Qunying Li, Zhijian J Clin Lab Anal Research Articles BACKGROUND: Renal biopsy remains the golden standard for diagnosing and monitoring IgA nephropathy (IgAN). Vascular endothelial growth factor A (VEGFA) was crucial for the survival of glomerular cells. Our aim was to screen the expression pattern of urinary, circulating and renal VEGFA in IgAN patients to reveal their relationship with renal pathology and outcomes. METHODS: Baseline VEGFA levels were determined with ELISA, real‐time PCR and immunohistochemistry. Associations between VEGFA expression and clinical–pathological parameters, and renal outcomes were evaluated. RESULTS: Compared with healthy controls, urinary VEGFA level was obviously elevated in IgAN patients (76.19 ± 63.67 pg/mg Cr vs 146.67 ± 232.71 pg/mg Cr, p = 0.0291) and not correlated with serum VEGFA level. Baseline urinary VEGFA was significantly associated with gender and tubular atrophy/interstitial fibrosis by stepwise multivariate regression analysis. Urinary VEGFA was higher in male patients accompanied with higher serum creatinine, larger proportion of hypertension and recurrent hematuria than in female patients. In the kidney of IgAN patients, VEGFA were robustly expressed in the parietal epithelial cells, podocytes, mesangial cells and tubular epithelial cells. After a follow‐up duration of 38.53 ± 27.14 months, IgAN patients with higher urinary VEGFA level were found to have a poorer renal outcome of renal replacement therapy (HR = 1.027, p = 0.037) or composite outcome (HR = 1.023, p = 0.039) after adjusting for confounders. CONCLUSIONS: Increased urinary VEGFA might reflect certain renal pathology and, although not fully specific, still could be served as a valuable noninvasive indicator in predicting renal progression of IgAN. John Wiley and Sons Inc. 2021-09-08 /pmc/articles/PMC8551689/ /pubmed/34498313 http://dx.doi.org/10.1002/jcla.23995 Text en © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Feng, Shaozhen Huang, Naya Xue, Miaorong Zhang, Puhua Zhong, Zhong Guo, Qunying Li, Zhijian Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title | Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title_full | Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title_fullStr | Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title_full_unstemmed | Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title_short | Association between urinary VEGFA and renal pathology of IgA nephropathy patients |
title_sort | association between urinary vegfa and renal pathology of iga nephropathy patients |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551689/ https://www.ncbi.nlm.nih.gov/pubmed/34498313 http://dx.doi.org/10.1002/jcla.23995 |
work_keys_str_mv | AT fengshaozhen associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT huangnaya associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT xuemiaorong associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT zhangpuhua associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT zhongzhong associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT guoqunying associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients AT lizhijian associationbetweenurinaryvegfaandrenalpathologyofiganephropathypatients |