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Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. The Coronavirus Disease 2019 (COVID-19) challenges health systems to quickly respond by developing new diagnostic strategies that contribute to identify...

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Detalles Bibliográficos
Autores principales: Tomas-Grau, Rodrigo Hernán, Ploper, Diego, Ávila, César Luis, Vera Pingitore, Esteban, Maldonado Galdeano, Carolina, Chaves, Silvina, Socias, Sergio Benjamín, Stagnetto, Agustín, Navarro, Silvia Adriana, Chahla, Rossana Elena, Aguilar López, Mónica, Llapur, Conrado Juan, Aznar, Patricia, Alcorta, María Elena, Costas, Dardo, Flores, Isolina, Heinze, Dar, Apfelbaum, Gabriela, Mostoslavsky, Raul, Mostoslavsky, Gustavo, Cazorla, Silvia Inés, Perdigón, Gabriela del Valle, Chehín, Rosana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551828/
https://www.ncbi.nlm.nih.gov/pubmed/34722566
http://dx.doi.org/10.3389/fmed.2021.720988
Descripción
Sumario:The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. The Coronavirus Disease 2019 (COVID-19) challenges health systems to quickly respond by developing new diagnostic strategies that contribute to identify infected individuals, monitor infections, perform contact-tracing, and limit the spread of the virus. In this brief report, we developed a highly sensitive, specific, and precise “In-House” ELISA to correctly discriminate previously SARS-CoV-2-infected and non-infected individuals and study population seroprevalence. Among 758 individuals evaluated for anti-SARS-CoV-2 serology in the province of Tucumán, Argentina, we found a weak correlation between antibodies elicited against the RBD, the receptor-binding domain of the Spike protein, and the nucleocapsid (N) antigens of this virus. Additionally, we detected mild levels of anti-RBD IgG antibodies in 33.6% of individuals diagnosed with COVID-19, while only 19% showed sufficient antibody titers to be considered as plasma donors. No differences in IgG anti-RBD titers were found between women and men, neither in between different age groups ranging from 18 to 60. Surprisingly, individuals from a high altitude village displayed elevated and longer lasting anti-RBD titers compared to those from a lower altitude city. To our knowledge, this is the first report correlating altitude with increased humoral immune response against SARS-CoV-2 infection.