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Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. The Coronavirus Disease 2019 (COVID-19) challenges health systems to quickly respond by developing new diagnostic strategies that contribute to identify...

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Autores principales: Tomas-Grau, Rodrigo Hernán, Ploper, Diego, Ávila, César Luis, Vera Pingitore, Esteban, Maldonado Galdeano, Carolina, Chaves, Silvina, Socias, Sergio Benjamín, Stagnetto, Agustín, Navarro, Silvia Adriana, Chahla, Rossana Elena, Aguilar López, Mónica, Llapur, Conrado Juan, Aznar, Patricia, Alcorta, María Elena, Costas, Dardo, Flores, Isolina, Heinze, Dar, Apfelbaum, Gabriela, Mostoslavsky, Raul, Mostoslavsky, Gustavo, Cazorla, Silvia Inés, Perdigón, Gabriela del Valle, Chehín, Rosana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551828/
https://www.ncbi.nlm.nih.gov/pubmed/34722566
http://dx.doi.org/10.3389/fmed.2021.720988
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author Tomas-Grau, Rodrigo Hernán
Ploper, Diego
Ávila, César Luis
Vera Pingitore, Esteban
Maldonado Galdeano, Carolina
Chaves, Silvina
Socias, Sergio Benjamín
Stagnetto, Agustín
Navarro, Silvia Adriana
Chahla, Rossana Elena
Aguilar López, Mónica
Llapur, Conrado Juan
Aznar, Patricia
Alcorta, María Elena
Costas, Dardo
Flores, Isolina
Heinze, Dar
Apfelbaum, Gabriela
Mostoslavsky, Raul
Mostoslavsky, Gustavo
Cazorla, Silvia Inés
Perdigón, Gabriela del Valle
Chehín, Rosana
author_facet Tomas-Grau, Rodrigo Hernán
Ploper, Diego
Ávila, César Luis
Vera Pingitore, Esteban
Maldonado Galdeano, Carolina
Chaves, Silvina
Socias, Sergio Benjamín
Stagnetto, Agustín
Navarro, Silvia Adriana
Chahla, Rossana Elena
Aguilar López, Mónica
Llapur, Conrado Juan
Aznar, Patricia
Alcorta, María Elena
Costas, Dardo
Flores, Isolina
Heinze, Dar
Apfelbaum, Gabriela
Mostoslavsky, Raul
Mostoslavsky, Gustavo
Cazorla, Silvia Inés
Perdigón, Gabriela del Valle
Chehín, Rosana
author_sort Tomas-Grau, Rodrigo Hernán
collection PubMed
description The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. The Coronavirus Disease 2019 (COVID-19) challenges health systems to quickly respond by developing new diagnostic strategies that contribute to identify infected individuals, monitor infections, perform contact-tracing, and limit the spread of the virus. In this brief report, we developed a highly sensitive, specific, and precise “In-House” ELISA to correctly discriminate previously SARS-CoV-2-infected and non-infected individuals and study population seroprevalence. Among 758 individuals evaluated for anti-SARS-CoV-2 serology in the province of Tucumán, Argentina, we found a weak correlation between antibodies elicited against the RBD, the receptor-binding domain of the Spike protein, and the nucleocapsid (N) antigens of this virus. Additionally, we detected mild levels of anti-RBD IgG antibodies in 33.6% of individuals diagnosed with COVID-19, while only 19% showed sufficient antibody titers to be considered as plasma donors. No differences in IgG anti-RBD titers were found between women and men, neither in between different age groups ranging from 18 to 60. Surprisingly, individuals from a high altitude village displayed elevated and longer lasting anti-RBD titers compared to those from a lower altitude city. To our knowledge, this is the first report correlating altitude with increased humoral immune response against SARS-CoV-2 infection.
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spelling pubmed-85518282021-10-29 Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA Tomas-Grau, Rodrigo Hernán Ploper, Diego Ávila, César Luis Vera Pingitore, Esteban Maldonado Galdeano, Carolina Chaves, Silvina Socias, Sergio Benjamín Stagnetto, Agustín Navarro, Silvia Adriana Chahla, Rossana Elena Aguilar López, Mónica Llapur, Conrado Juan Aznar, Patricia Alcorta, María Elena Costas, Dardo Flores, Isolina Heinze, Dar Apfelbaum, Gabriela Mostoslavsky, Raul Mostoslavsky, Gustavo Cazorla, Silvia Inés Perdigón, Gabriela del Valle Chehín, Rosana Front Med (Lausanne) Medicine The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. The Coronavirus Disease 2019 (COVID-19) challenges health systems to quickly respond by developing new diagnostic strategies that contribute to identify infected individuals, monitor infections, perform contact-tracing, and limit the spread of the virus. In this brief report, we developed a highly sensitive, specific, and precise “In-House” ELISA to correctly discriminate previously SARS-CoV-2-infected and non-infected individuals and study population seroprevalence. Among 758 individuals evaluated for anti-SARS-CoV-2 serology in the province of Tucumán, Argentina, we found a weak correlation between antibodies elicited against the RBD, the receptor-binding domain of the Spike protein, and the nucleocapsid (N) antigens of this virus. Additionally, we detected mild levels of anti-RBD IgG antibodies in 33.6% of individuals diagnosed with COVID-19, while only 19% showed sufficient antibody titers to be considered as plasma donors. No differences in IgG anti-RBD titers were found between women and men, neither in between different age groups ranging from 18 to 60. Surprisingly, individuals from a high altitude village displayed elevated and longer lasting anti-RBD titers compared to those from a lower altitude city. To our knowledge, this is the first report correlating altitude with increased humoral immune response against SARS-CoV-2 infection. Frontiers Media S.A. 2021-10-14 /pmc/articles/PMC8551828/ /pubmed/34722566 http://dx.doi.org/10.3389/fmed.2021.720988 Text en Copyright © 2021 Tomas-Grau, Ploper, Ávila, Vera Pingitore, Maldonado Galdeano, Chaves, Socias, Stagnetto, Navarro, Chahla, Aguilar López, Llapur, Aznar, Alcorta, Costas, Flores, Heinze, Apfelbaum, Mostoslavsky, Mostoslavsky, Cazorla, Perdigón and Chehín. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Tomas-Grau, Rodrigo Hernán
Ploper, Diego
Ávila, César Luis
Vera Pingitore, Esteban
Maldonado Galdeano, Carolina
Chaves, Silvina
Socias, Sergio Benjamín
Stagnetto, Agustín
Navarro, Silvia Adriana
Chahla, Rossana Elena
Aguilar López, Mónica
Llapur, Conrado Juan
Aznar, Patricia
Alcorta, María Elena
Costas, Dardo
Flores, Isolina
Heinze, Dar
Apfelbaum, Gabriela
Mostoslavsky, Raul
Mostoslavsky, Gustavo
Cazorla, Silvia Inés
Perdigón, Gabriela del Valle
Chehín, Rosana
Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title_full Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title_fullStr Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title_full_unstemmed Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title_short Elevated Humoral Immune Response to SARS-CoV-2 at High Altitudes Revealed by an Anti-RBD “In-House” ELISA
title_sort elevated humoral immune response to sars-cov-2 at high altitudes revealed by an anti-rbd “in-house” elisa
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551828/
https://www.ncbi.nlm.nih.gov/pubmed/34722566
http://dx.doi.org/10.3389/fmed.2021.720988
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