Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications

Cytotoxic natural killer cells kill tumors and infected cells. We carried out CRISPR-based gene editing and transcriptional regulation in hard-to-manipulate NK-92 cells. NK-92-based therapies were found to be safe and efficacious in preclinical studies of cancers. Here, we have pioneered the generat...

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Autores principales: Kumar, Anil, Lee, Sung June, Liu, Qiao, Chan, Anthony K.N., Pokharel, Sheela Pangeni, Yu, Jianhua, Chen, Chun-Wei, Swaminathan, Srividya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551845/
https://www.ncbi.nlm.nih.gov/pubmed/34746857
http://dx.doi.org/10.1016/j.xpro.2021.100874
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author Kumar, Anil
Lee, Sung June
Liu, Qiao
Chan, Anthony K.N.
Pokharel, Sheela Pangeni
Yu, Jianhua
Chen, Chun-Wei
Swaminathan, Srividya
author_facet Kumar, Anil
Lee, Sung June
Liu, Qiao
Chan, Anthony K.N.
Pokharel, Sheela Pangeni
Yu, Jianhua
Chen, Chun-Wei
Swaminathan, Srividya
author_sort Kumar, Anil
collection PubMed
description Cytotoxic natural killer cells kill tumors and infected cells. We carried out CRISPR-based gene editing and transcriptional regulation in hard-to-manipulate NK-92 cells. NK-92-based therapies were found to be safe and efficacious in preclinical studies of cancers. Here, we have pioneered the generation and validation of NK-92 cells constitutively expressing Cas9 or dCas9 for knockout (CRISPRko), transcriptional activation (CRISPRa), or transcriptional repression (CRISPRi) of genes. Our CRISPR-engineered NK-92 cell platforms can be modified for research and off-the-shelf therapeutic applications.
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spelling pubmed-85518452021-11-04 Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications Kumar, Anil Lee, Sung June Liu, Qiao Chan, Anthony K.N. Pokharel, Sheela Pangeni Yu, Jianhua Chen, Chun-Wei Swaminathan, Srividya STAR Protoc Protocol Cytotoxic natural killer cells kill tumors and infected cells. We carried out CRISPR-based gene editing and transcriptional regulation in hard-to-manipulate NK-92 cells. NK-92-based therapies were found to be safe and efficacious in preclinical studies of cancers. Here, we have pioneered the generation and validation of NK-92 cells constitutively expressing Cas9 or dCas9 for knockout (CRISPRko), transcriptional activation (CRISPRa), or transcriptional repression (CRISPRi) of genes. Our CRISPR-engineered NK-92 cell platforms can be modified for research and off-the-shelf therapeutic applications. Elsevier 2021-10-21 /pmc/articles/PMC8551845/ /pubmed/34746857 http://dx.doi.org/10.1016/j.xpro.2021.100874 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kumar, Anil
Lee, Sung June
Liu, Qiao
Chan, Anthony K.N.
Pokharel, Sheela Pangeni
Yu, Jianhua
Chen, Chun-Wei
Swaminathan, Srividya
Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title_full Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title_fullStr Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title_full_unstemmed Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title_short Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
title_sort generation and validation of crispr-engineered human natural killer cell lines for research and therapeutic applications
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551845/
https://www.ncbi.nlm.nih.gov/pubmed/34746857
http://dx.doi.org/10.1016/j.xpro.2021.100874
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