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Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media

This protocol describes how to obtain human astrocytes from human-induced pluripotent stem cells (hiPSCs) in chemically defined media, without the use of fetal bovine serum (FBS). FBS eases the differentiation of astrocytes but also deeply alters their phenotype, as compared with their in vivo count...

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Detalles Bibliográficos
Autores principales:	Perriot, Sylvain, Canales, Mathieu, Mathias, Amandine, Du Pasquier, Renaud
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551928/ https://www.ncbi.nlm.nih.gov/pubmed/34746863 http://dx.doi.org/10.1016/j.xpro.2021.100902

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