Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells

Several kidney diseases including congenital nephrotic syndrome, Alport syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human podocytopathies may be modeled in either primary or immortalized podocyte cell lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are...

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Detalles Bibliográficos
Autores principales: Bejoy, Julie, Qian, Eddie Spencer, Woodard, Lauren Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551929/
https://www.ncbi.nlm.nih.gov/pubmed/34746862
http://dx.doi.org/10.1016/j.xpro.2021.100898
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author Bejoy, Julie
Qian, Eddie Spencer
Woodard, Lauren Elizabeth
author_facet Bejoy, Julie
Qian, Eddie Spencer
Woodard, Lauren Elizabeth
author_sort Bejoy, Julie
collection PubMed
description Several kidney diseases including congenital nephrotic syndrome, Alport syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human podocytopathies may be modeled in either primary or immortalized podocyte cell lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are a source of human podocytes, but the existing protocols have variable efficiency and expensive media components. We developed an accelerated, feeder-free protocol for deriving functional, mature podocytes from hiPSCs in only 12 days, saving time and money compared with other approaches.
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spelling pubmed-85519292021-11-04 Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells Bejoy, Julie Qian, Eddie Spencer Woodard, Lauren Elizabeth STAR Protoc Protocol Several kidney diseases including congenital nephrotic syndrome, Alport syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human podocytopathies may be modeled in either primary or immortalized podocyte cell lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are a source of human podocytes, but the existing protocols have variable efficiency and expensive media components. We developed an accelerated, feeder-free protocol for deriving functional, mature podocytes from hiPSCs in only 12 days, saving time and money compared with other approaches. Elsevier 2021-10-20 /pmc/articles/PMC8551929/ /pubmed/34746862 http://dx.doi.org/10.1016/j.xpro.2021.100898 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bejoy, Julie
Qian, Eddie Spencer
Woodard, Lauren Elizabeth
Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title_full Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title_fullStr Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title_full_unstemmed Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title_short Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
title_sort accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551929/
https://www.ncbi.nlm.nih.gov/pubmed/34746862
http://dx.doi.org/10.1016/j.xpro.2021.100898
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AT woodardlaurenelizabeth acceleratedprotocolforthedifferentiationofpodocytesfromhumanpluripotentstemcells

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