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Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma
Tumor endothelial marker 8 (TEM8), also known as ANTXR1, was highly expressed in cancers, and was identified as a biomarker for early diagnosis and prognosis in some cancers. However, the clinical role and molecular mechanisms of TEM8 in lung adenocarcinoma (LUAD) are still unclear. The present stud...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552045/ https://www.ncbi.nlm.nih.gov/pubmed/34722259 http://dx.doi.org/10.3389/fonc.2021.712371 |
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author | Ding, Chen Liu, Jun Zhang, Jiali Wan, Yang Hu, Linhui Charwudzi, Alice Zhan, Heqin Meng, Ye Zheng, Huimin Wang, HuiPing Wang, Youliang Gao, Lihua Hu, Xianwen Li, Jingrong Xiong, Shudao |
author_facet | Ding, Chen Liu, Jun Zhang, Jiali Wan, Yang Hu, Linhui Charwudzi, Alice Zhan, Heqin Meng, Ye Zheng, Huimin Wang, HuiPing Wang, Youliang Gao, Lihua Hu, Xianwen Li, Jingrong Xiong, Shudao |
author_sort | Ding, Chen |
collection | PubMed |
description | Tumor endothelial marker 8 (TEM8), also known as ANTXR1, was highly expressed in cancers, and was identified as a biomarker for early diagnosis and prognosis in some cancers. However, the clinical role and molecular mechanisms of TEM8 in lung adenocarcinoma (LUAD) are still unclear. The present study aimed to explore its clinical value and the molecular mechanisms of TEM8 underlying the progression of LUAD. Our study found the elevation of TEM8 in LUAD cell lines and tissues. What’s more, we observed that the TEM8 expression level was associated with tumor size, primary tumor, and AJCC stage, and LUAD patients with high TEM8 expression usually have a poor prognosis. Then, we conducted a series of experiments by the strategy of loss-of-function and gain-of-function, and our results suggested that the knockdown of TEM8 suppressed proliferation, migration, and invasion and induced apoptosis in LUAD whereas overexpression of TEM8 had the opposite effect. Molecular mechanistic investigation showed that TEM8 exerted its promoting effects mainly through activating the Wnt/β-catenin signaling pathway. In short, our findings suggested that TEM8 played a crucial role in the progression of LUAD by activating the Wnt/β-catenin signaling pathway and could serve as a potential therapeutic target for LUAD. |
format | Online Article Text |
id | pubmed-8552045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85520452021-10-29 Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma Ding, Chen Liu, Jun Zhang, Jiali Wan, Yang Hu, Linhui Charwudzi, Alice Zhan, Heqin Meng, Ye Zheng, Huimin Wang, HuiPing Wang, Youliang Gao, Lihua Hu, Xianwen Li, Jingrong Xiong, Shudao Front Oncol Oncology Tumor endothelial marker 8 (TEM8), also known as ANTXR1, was highly expressed in cancers, and was identified as a biomarker for early diagnosis and prognosis in some cancers. However, the clinical role and molecular mechanisms of TEM8 in lung adenocarcinoma (LUAD) are still unclear. The present study aimed to explore its clinical value and the molecular mechanisms of TEM8 underlying the progression of LUAD. Our study found the elevation of TEM8 in LUAD cell lines and tissues. What’s more, we observed that the TEM8 expression level was associated with tumor size, primary tumor, and AJCC stage, and LUAD patients with high TEM8 expression usually have a poor prognosis. Then, we conducted a series of experiments by the strategy of loss-of-function and gain-of-function, and our results suggested that the knockdown of TEM8 suppressed proliferation, migration, and invasion and induced apoptosis in LUAD whereas overexpression of TEM8 had the opposite effect. Molecular mechanistic investigation showed that TEM8 exerted its promoting effects mainly through activating the Wnt/β-catenin signaling pathway. In short, our findings suggested that TEM8 played a crucial role in the progression of LUAD by activating the Wnt/β-catenin signaling pathway and could serve as a potential therapeutic target for LUAD. Frontiers Media S.A. 2021-10-14 /pmc/articles/PMC8552045/ /pubmed/34722259 http://dx.doi.org/10.3389/fonc.2021.712371 Text en Copyright © 2021 Ding, Liu, Zhang, Wan, Hu, Charwudzi, Zhan, Meng, Zheng, Wang, Wang, Gao, Hu, Li and Xiong https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Ding, Chen Liu, Jun Zhang, Jiali Wan, Yang Hu, Linhui Charwudzi, Alice Zhan, Heqin Meng, Ye Zheng, Huimin Wang, HuiPing Wang, Youliang Gao, Lihua Hu, Xianwen Li, Jingrong Xiong, Shudao Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title | Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title_full | Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title_fullStr | Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title_full_unstemmed | Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title_short | Tumor Endothelial Marker 8 Promotes Proliferation and Metastasis via the Wnt/β-Catenin Signaling Pathway in Lung Adenocarcinoma |
title_sort | tumor endothelial marker 8 promotes proliferation and metastasis via the wnt/β-catenin signaling pathway in lung adenocarcinoma |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552045/ https://www.ncbi.nlm.nih.gov/pubmed/34722259 http://dx.doi.org/10.3389/fonc.2021.712371 |
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