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The impact of viral mutations on recognition by SARS-CoV-2 specific T cells
We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552693/ https://www.ncbi.nlm.nih.gov/pubmed/34729465 http://dx.doi.org/10.1016/j.isci.2021.103353 |
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| author | de Silva, Thushan I. Liu, Guihai Lindsey, Benjamin B. Dong, Danning Moore, Shona C. Hsu, Nienyun Sharon Shah, Dhruv Wellington, Dannielle Mentzer, Alexander J. Angyal, Adrienn Brown, Rebecca Parker, Matthew D. Ying, Zixi Yao, Xuan Turtle, Lance Dunachie, Susanna Maini, Mala K. Ogg, Graham Knight, Julian C. Peng, Yanchun Rowland-Jones, Sarah L. Dong, Tao |
| author_facet | de Silva, Thushan I. Liu, Guihai Lindsey, Benjamin B. Dong, Danning Moore, Shona C. Hsu, Nienyun Sharon Shah, Dhruv Wellington, Dannielle Mentzer, Alexander J. Angyal, Adrienn Brown, Rebecca Parker, Matthew D. Ying, Zixi Yao, Xuan Turtle, Lance Dunachie, Susanna Maini, Mala K. Ogg, Graham Knight, Julian C. Peng, Yanchun Rowland-Jones, Sarah L. Dong, Tao |
| author_sort | de Silva, Thushan I. |
| collection | PubMed |
| description | We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY(207-215); due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF(9-17); and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK(361-369). CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity. |
| format | Online Article Text |
| id | pubmed-8552693 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-85526932021-10-29 The impact of viral mutations on recognition by SARS-CoV-2 specific T cells de Silva, Thushan I. Liu, Guihai Lindsey, Benjamin B. Dong, Danning Moore, Shona C. Hsu, Nienyun Sharon Shah, Dhruv Wellington, Dannielle Mentzer, Alexander J. Angyal, Adrienn Brown, Rebecca Parker, Matthew D. Ying, Zixi Yao, Xuan Turtle, Lance Dunachie, Susanna Maini, Mala K. Ogg, Graham Knight, Julian C. Peng, Yanchun Rowland-Jones, Sarah L. Dong, Tao iScience Article We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY(207-215); due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF(9-17); and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK(361-369). CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity. Elsevier 2021-10-28 /pmc/articles/PMC8552693/ /pubmed/34729465 http://dx.doi.org/10.1016/j.isci.2021.103353 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article de Silva, Thushan I. Liu, Guihai Lindsey, Benjamin B. Dong, Danning Moore, Shona C. Hsu, Nienyun Sharon Shah, Dhruv Wellington, Dannielle Mentzer, Alexander J. Angyal, Adrienn Brown, Rebecca Parker, Matthew D. Ying, Zixi Yao, Xuan Turtle, Lance Dunachie, Susanna Maini, Mala K. Ogg, Graham Knight, Julian C. Peng, Yanchun Rowland-Jones, Sarah L. Dong, Tao The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title_full | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title_fullStr | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title_full_unstemmed | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title_short | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
| title_sort | impact of viral mutations on recognition by sars-cov-2 specific t cells |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552693/ https://www.ncbi.nlm.nih.gov/pubmed/34729465 http://dx.doi.org/10.1016/j.isci.2021.103353 |
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