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Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model

The host defense peptide caerin 1.9 was originally isolated from skin secretions of an Australian tree frog and inhibits the growth of a wide range of bacteria in vitro. In this study, we demonstrated that caerin 1.9 shows high bioactivity against several bacteria strains, such as Staphylococcus aur...

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Autores principales: Chen, Shu, Zhang, Pingping, Xiao, Liyin, Liu, Ying, Wu, Kuihai, Ni, Guoying, Li, Hejie, Wang, Tianfang, Wu, Xiaolian, Chen, Guoqiang, Liu, Xiaosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552723/
https://www.ncbi.nlm.nih.gov/pubmed/34259550
http://dx.doi.org/10.1128/spectrum.00051-21
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author Chen, Shu
Zhang, Pingping
Xiao, Liyin
Liu, Ying
Wu, Kuihai
Ni, Guoying
Li, Hejie
Wang, Tianfang
Wu, Xiaolian
Chen, Guoqiang
Liu, Xiaosong
author_facet Chen, Shu
Zhang, Pingping
Xiao, Liyin
Liu, Ying
Wu, Kuihai
Ni, Guoying
Li, Hejie
Wang, Tianfang
Wu, Xiaolian
Chen, Guoqiang
Liu, Xiaosong
author_sort Chen, Shu
collection PubMed
description The host defense peptide caerin 1.9 was originally isolated from skin secretions of an Australian tree frog and inhibits the growth of a wide range of bacteria in vitro. In this study, we demonstrated that caerin 1.9 shows high bioactivity against several bacteria strains, such as Staphylococcus aureus, Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus haemolyticus in vitro. Importantly, unlike the antibiotic Tazocin, caerin 1.9 does not induce bacterial resistance after 30 rounds of in vitro culture. Moreover, caerin 1.1, another peptide of the caerin family, has an additive antibacterial effect when used together with caerin 1.9. Furthermore, caerin 1.1 and 1.9 prepared in the form of a temperature-sensitive gel inhibit MRSA growth in a skin bacterial infection model of two murine strains. These results indicate that caerin 1.1 and 1.9 peptides could be considered an alternative for conventional antibiotics. IMPORTANCE Antibiotic-resistant bacteria cause severe problems in the clinic. We show in our paper that two short peptides isolated from an Australian frog and prepared in the form of a gel are able to inhibit the growth of antibiotic-resistant bacteria in mice, and, unlike antibiotics, these peptides do not lead to the development of peptide-resistant bacteria strains.
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spelling pubmed-85527232021-11-08 Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model Chen, Shu Zhang, Pingping Xiao, Liyin Liu, Ying Wu, Kuihai Ni, Guoying Li, Hejie Wang, Tianfang Wu, Xiaolian Chen, Guoqiang Liu, Xiaosong Microbiol Spectr Research Article The host defense peptide caerin 1.9 was originally isolated from skin secretions of an Australian tree frog and inhibits the growth of a wide range of bacteria in vitro. In this study, we demonstrated that caerin 1.9 shows high bioactivity against several bacteria strains, such as Staphylococcus aureus, Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus haemolyticus in vitro. Importantly, unlike the antibiotic Tazocin, caerin 1.9 does not induce bacterial resistance after 30 rounds of in vitro culture. Moreover, caerin 1.1, another peptide of the caerin family, has an additive antibacterial effect when used together with caerin 1.9. Furthermore, caerin 1.1 and 1.9 prepared in the form of a temperature-sensitive gel inhibit MRSA growth in a skin bacterial infection model of two murine strains. These results indicate that caerin 1.1 and 1.9 peptides could be considered an alternative for conventional antibiotics. IMPORTANCE Antibiotic-resistant bacteria cause severe problems in the clinic. We show in our paper that two short peptides isolated from an Australian frog and prepared in the form of a gel are able to inhibit the growth of antibiotic-resistant bacteria in mice, and, unlike antibiotics, these peptides do not lead to the development of peptide-resistant bacteria strains. American Society for Microbiology 2021-07-14 /pmc/articles/PMC8552723/ /pubmed/34259550 http://dx.doi.org/10.1128/spectrum.00051-21 Text en Copyright © 2021 Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Chen, Shu
Zhang, Pingping
Xiao, Liyin
Liu, Ying
Wu, Kuihai
Ni, Guoying
Li, Hejie
Wang, Tianfang
Wu, Xiaolian
Chen, Guoqiang
Liu, Xiaosong
Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title_full Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title_fullStr Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title_full_unstemmed Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title_short Caerin 1.1 and 1.9 Peptides from Australian Tree Frog Inhibit Antibiotic-Resistant Bacteria Growth in a Murine Skin Infection Model
title_sort caerin 1.1 and 1.9 peptides from australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552723/
https://www.ncbi.nlm.nih.gov/pubmed/34259550
http://dx.doi.org/10.1128/spectrum.00051-21
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