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A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the co...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553343/ https://www.ncbi.nlm.nih.gov/pubmed/34633289 http://dx.doi.org/10.7554/eLife.70471 |
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author | Huang, Hui Lin, Youpei Ma, Wenrui Liu, Jiannan Han, Jing Hu, Xiaoyi Tang, Meilin Yan, Shiqiang Abudupataer, Mieradilijiang Zhang, Chenping Gao, Qiang Zhang, Weijia |
author_facet | Huang, Hui Lin, Youpei Ma, Wenrui Liu, Jiannan Han, Jing Hu, Xiaoyi Tang, Meilin Yan, Shiqiang Abudupataer, Mieradilijiang Zhang, Chenping Gao, Qiang Zhang, Weijia |
author_sort | Huang, Hui |
collection | PubMed |
description | To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins. |
format | Online Article Text |
id | pubmed-8553343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-85533432021-10-29 A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia Huang, Hui Lin, Youpei Ma, Wenrui Liu, Jiannan Han, Jing Hu, Xiaoyi Tang, Meilin Yan, Shiqiang Abudupataer, Mieradilijiang Zhang, Chenping Gao, Qiang Zhang, Weijia eLife Biochemistry and Chemical Biology To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins. eLife Sciences Publications, Ltd 2021-10-11 /pmc/articles/PMC8553343/ /pubmed/34633289 http://dx.doi.org/10.7554/eLife.70471 Text en © 2021, Huang et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Huang, Hui Lin, Youpei Ma, Wenrui Liu, Jiannan Han, Jing Hu, Xiaoyi Tang, Meilin Yan, Shiqiang Abudupataer, Mieradilijiang Zhang, Chenping Gao, Qiang Zhang, Weijia A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title | A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title_full | A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title_fullStr | A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title_full_unstemmed | A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title_short | A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
title_sort | pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553343/ https://www.ncbi.nlm.nih.gov/pubmed/34633289 http://dx.doi.org/10.7554/eLife.70471 |
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