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A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia

To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the co...

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Autores principales: Huang, Hui, Lin, Youpei, Ma, Wenrui, Liu, Jiannan, Han, Jing, Hu, Xiaoyi, Tang, Meilin, Yan, Shiqiang, Abudupataer, Mieradilijiang, Zhang, Chenping, Gao, Qiang, Zhang, Weijia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553343/
https://www.ncbi.nlm.nih.gov/pubmed/34633289
http://dx.doi.org/10.7554/eLife.70471
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author Huang, Hui
Lin, Youpei
Ma, Wenrui
Liu, Jiannan
Han, Jing
Hu, Xiaoyi
Tang, Meilin
Yan, Shiqiang
Abudupataer, Mieradilijiang
Zhang, Chenping
Gao, Qiang
Zhang, Weijia
author_facet Huang, Hui
Lin, Youpei
Ma, Wenrui
Liu, Jiannan
Han, Jing
Hu, Xiaoyi
Tang, Meilin
Yan, Shiqiang
Abudupataer, Mieradilijiang
Zhang, Chenping
Gao, Qiang
Zhang, Weijia
author_sort Huang, Hui
collection PubMed
description To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins.
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spelling pubmed-85533432021-10-29 A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia Huang, Hui Lin, Youpei Ma, Wenrui Liu, Jiannan Han, Jing Hu, Xiaoyi Tang, Meilin Yan, Shiqiang Abudupataer, Mieradilijiang Zhang, Chenping Gao, Qiang Zhang, Weijia eLife Biochemistry and Chemical Biology To assure complete tumor removal, frozen section analysis is the most common procedure for intraoperative pathological assessment of resected tumor margins. However, during one operation, multiple biopsies may be sent for examination, but only few of them are made into cryosections because of the complex preparation protocols and time-consuming pathological analysis, which potentially increases the risk of overlooking tumor involvement. Here, we propose a fluorescence-based pre-screening strategy that allows high-throughput, convenient, and fast gross assessment of resected tumor margins. A dual-activatable cationic fluorescent molecular rotor was developed to specifically illuminate live tumor cells’ cytoplasm by emitting two different fluorescence signals in response to elevations in hypoxia-induced nitroreductase (a biochemical marker) and cytoplasmic viscosity (a biophysical marker), two characteristics of cancer cells. The ability of the fluorescent molecular rotor in detecting tumor cells was evaluated in mouse and human specimens of multiple tissues by comparing with hematoxylin and eosin staining. Importantly, the fluorescent molecular rotor achieved 100 % specificity in discriminating lung and liver cancers from normal tissue, allowing pre-screening of the tumor-free surgical margins and promoting clinical decision. Altogether, this type of fluorescent molecular rotor and the proposed strategy may serve as a new option to facilitate intraoperative assessment of resected tumor margins. eLife Sciences Publications, Ltd 2021-10-11 /pmc/articles/PMC8553343/ /pubmed/34633289 http://dx.doi.org/10.7554/eLife.70471 Text en © 2021, Huang et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Huang, Hui
Lin, Youpei
Ma, Wenrui
Liu, Jiannan
Han, Jing
Hu, Xiaoyi
Tang, Meilin
Yan, Shiqiang
Abudupataer, Mieradilijiang
Zhang, Chenping
Gao, Qiang
Zhang, Weijia
A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title_full A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title_fullStr A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title_full_unstemmed A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title_short A pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
title_sort pre-screening strategy to assess resected tumor margins by imaging cytoplasmic viscosity and hypoxia
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553343/
https://www.ncbi.nlm.nih.gov/pubmed/34633289
http://dx.doi.org/10.7554/eLife.70471
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