Cargando…
Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria
Polyphosphate [poly(P)] is described as a homopolymer of inorganic phosphates. Nicotinamide adenine dinucleotide kinase (NAD kinase) catalyzes the phosphorylation of NAD(+) to NADP(+) in the presence of ATP (ATP-NAD kinase). Novel NAD kinase that explicitly phosphorylates NAD(+) to NADP(+) using pol...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japan Academy
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553519/ https://www.ncbi.nlm.nih.gov/pubmed/34629356 http://dx.doi.org/10.2183/pjab.97.024 |
_version_ | 1784591602004525056 |
---|---|
author | MURATA, Kousaku |
author_facet | MURATA, Kousaku |
author_sort | MURATA, Kousaku |
collection | PubMed |
description | Polyphosphate [poly(P)] is described as a homopolymer of inorganic phosphates. Nicotinamide adenine dinucleotide kinase (NAD kinase) catalyzes the phosphorylation of NAD(+) to NADP(+) in the presence of ATP (ATP-NAD kinase). Novel NAD kinase that explicitly phosphorylates NAD(+) to NADP(+) using poly(P), besides ATP [ATP/poly(P)-NAD kinase], was found in bacteria, in particular, Gram-positive bacteria, and the gene encoding ATP/poly(P)-NAD kinase was also newly identified in Mycobacterium tuberculosis H37Rv. Both NAD kinases required multi-homopolymeric structures for activity expression. The enzymatic and genetic results, combined with their primary and tertiary structures, have led to the discovery of a long-awaited human mitochondrial NAD kinase. This discovery showed that the NAD kinase is a bacterial type of ATP/poly(P)-NAD kinase. These pioneering findings, i.e., ATP/poly(P)-NAD kinase, NAD kinase gene, and human mitochondrial NAD kinase, have significantly enhanced research on the biochemistry, molecular biology, and evolutionary biology of NAD kinase, mitochondria, and poly(P), including some biotechnological knowledge applicable to NADP(+) production. |
format | Online Article Text |
id | pubmed-8553519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Japan Academy |
record_format | MEDLINE/PubMed |
spelling | pubmed-85535192021-11-02 Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria MURATA, Kousaku Proc Jpn Acad Ser B Phys Biol Sci Review Polyphosphate [poly(P)] is described as a homopolymer of inorganic phosphates. Nicotinamide adenine dinucleotide kinase (NAD kinase) catalyzes the phosphorylation of NAD(+) to NADP(+) in the presence of ATP (ATP-NAD kinase). Novel NAD kinase that explicitly phosphorylates NAD(+) to NADP(+) using poly(P), besides ATP [ATP/poly(P)-NAD kinase], was found in bacteria, in particular, Gram-positive bacteria, and the gene encoding ATP/poly(P)-NAD kinase was also newly identified in Mycobacterium tuberculosis H37Rv. Both NAD kinases required multi-homopolymeric structures for activity expression. The enzymatic and genetic results, combined with their primary and tertiary structures, have led to the discovery of a long-awaited human mitochondrial NAD kinase. This discovery showed that the NAD kinase is a bacterial type of ATP/poly(P)-NAD kinase. These pioneering findings, i.e., ATP/poly(P)-NAD kinase, NAD kinase gene, and human mitochondrial NAD kinase, have significantly enhanced research on the biochemistry, molecular biology, and evolutionary biology of NAD kinase, mitochondria, and poly(P), including some biotechnological knowledge applicable to NADP(+) production. The Japan Academy 2021-10-11 /pmc/articles/PMC8553519/ /pubmed/34629356 http://dx.doi.org/10.2183/pjab.97.024 Text en © 2021 The Japan Academy https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review MURATA, Kousaku Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title | Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title_full | Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title_fullStr | Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title_full_unstemmed | Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title_short | Polyphosphate-dependent nicotinamide adenine dinucleotide (NAD) kinase: A novel missing link in human mitochondria |
title_sort | polyphosphate-dependent nicotinamide adenine dinucleotide (nad) kinase: a novel missing link in human mitochondria |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553519/ https://www.ncbi.nlm.nih.gov/pubmed/34629356 http://dx.doi.org/10.2183/pjab.97.024 |
work_keys_str_mv | AT muratakousaku polyphosphatedependentnicotinamideadeninedinucleotidenadkinaseanovelmissinglinkinhumanmitochondria |