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Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human

Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence...

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Autores principales: Bienert, Michaela, Habib, Pardes, Buck, Volker, Classen-Linke, Irmgard, Skoblo, Roman, Rösing, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553705/
https://www.ncbi.nlm.nih.gov/pubmed/33903941
http://dx.doi.org/10.1007/s00404-021-06031-9
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author Bienert, Michaela
Habib, Pardes
Buck, Volker
Classen-Linke, Irmgard
Skoblo, Roman
Rösing, Benjamin
author_facet Bienert, Michaela
Habib, Pardes
Buck, Volker
Classen-Linke, Irmgard
Skoblo, Roman
Rösing, Benjamin
author_sort Bienert, Michaela
collection PubMed
description Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART.
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spelling pubmed-85537052021-11-04 Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human Bienert, Michaela Habib, Pardes Buck, Volker Classen-Linke, Irmgard Skoblo, Roman Rösing, Benjamin Arch Gynecol Obstet Gynecologic Endocrinology and Reproductive Medicine Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART. Springer Berlin Heidelberg 2021-04-26 2021 /pmc/articles/PMC8553705/ /pubmed/33903941 http://dx.doi.org/10.1007/s00404-021-06031-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Gynecologic Endocrinology and Reproductive Medicine
Bienert, Michaela
Habib, Pardes
Buck, Volker
Classen-Linke, Irmgard
Skoblo, Roman
Rösing, Benjamin
Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title_full Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title_fullStr Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title_full_unstemmed Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title_short Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
title_sort intrauterine hcg application increases expression of endothelial cell–cell adhesion molecules in human
topic Gynecologic Endocrinology and Reproductive Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553705/
https://www.ncbi.nlm.nih.gov/pubmed/33903941
http://dx.doi.org/10.1007/s00404-021-06031-9
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