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Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human
Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553705/ https://www.ncbi.nlm.nih.gov/pubmed/33903941 http://dx.doi.org/10.1007/s00404-021-06031-9 |
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author | Bienert, Michaela Habib, Pardes Buck, Volker Classen-Linke, Irmgard Skoblo, Roman Rösing, Benjamin |
author_facet | Bienert, Michaela Habib, Pardes Buck, Volker Classen-Linke, Irmgard Skoblo, Roman Rösing, Benjamin |
author_sort | Bienert, Michaela |
collection | PubMed |
description | Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART. |
format | Online Article Text |
id | pubmed-8553705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-85537052021-11-04 Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human Bienert, Michaela Habib, Pardes Buck, Volker Classen-Linke, Irmgard Skoblo, Roman Rösing, Benjamin Arch Gynecol Obstet Gynecologic Endocrinology and Reproductive Medicine Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART. Springer Berlin Heidelberg 2021-04-26 2021 /pmc/articles/PMC8553705/ /pubmed/33903941 http://dx.doi.org/10.1007/s00404-021-06031-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Gynecologic Endocrinology and Reproductive Medicine Bienert, Michaela Habib, Pardes Buck, Volker Classen-Linke, Irmgard Skoblo, Roman Rösing, Benjamin Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title | Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title_full | Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title_fullStr | Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title_full_unstemmed | Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title_short | Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human |
title_sort | intrauterine hcg application increases expression of endothelial cell–cell adhesion molecules in human |
topic | Gynecologic Endocrinology and Reproductive Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553705/ https://www.ncbi.nlm.nih.gov/pubmed/33903941 http://dx.doi.org/10.1007/s00404-021-06031-9 |
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