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Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin

Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin....

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Autores principales: Cuddihy, Joshua, Wu, Gongjie, Ho, Laptin, Kudo, Hiromi, Dannhorn, Andreas, Mandalia, Sundhiya, Collins, Declan, Weir, Justin, Spencer, Ashley, Vizcaychipi, Marcela, Takats, Zoltan, Nagy, Istvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553775/
https://www.ncbi.nlm.nih.gov/pubmed/34711882
http://dx.doi.org/10.1038/s41598-021-00644-5
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author Cuddihy, Joshua
Wu, Gongjie
Ho, Laptin
Kudo, Hiromi
Dannhorn, Andreas
Mandalia, Sundhiya
Collins, Declan
Weir, Justin
Spencer, Ashley
Vizcaychipi, Marcela
Takats, Zoltan
Nagy, Istvan
author_facet Cuddihy, Joshua
Wu, Gongjie
Ho, Laptin
Kudo, Hiromi
Dannhorn, Andreas
Mandalia, Sundhiya
Collins, Declan
Weir, Justin
Spencer, Ashley
Vizcaychipi, Marcela
Takats, Zoltan
Nagy, Istvan
author_sort Cuddihy, Joshua
collection PubMed
description Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin. LDH activity was visualised in injured and uninjured skin from 38 sub-acute burn patients. LDH activity aided the identification of spatially correlating immunocompetent cells in a sub-group of six patients. Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) was used to describe relative lactate and pyruvate abundance in burned and uninjured tissue. LDH activity was significantly increased in the middle and deep regions of burnt skin compared with superficial areas in burnt skin and uninjured tissue and positively correlated with post-burn time. Regions of increased LDH activity showed high pyruvate and low lactate abundance when examined with DESI-MSI. Areas of increased LDH activity exhibited cellular infiltration, including CD3 + and CD4 + T-lymphocytes and CD68 + macrophages. Our data demonstrate a steady increase in functional LDH activity in sub-acute burn wounds linked to cellular infiltration. The cell types associated are related to tissue restructuring and inflammation. This region in burn wounds is likely the focus of dysregulated inflammation and hypermetabolism.
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spelling pubmed-85537752021-11-01 Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin Cuddihy, Joshua Wu, Gongjie Ho, Laptin Kudo, Hiromi Dannhorn, Andreas Mandalia, Sundhiya Collins, Declan Weir, Justin Spencer, Ashley Vizcaychipi, Marcela Takats, Zoltan Nagy, Istvan Sci Rep Article Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin. LDH activity was visualised in injured and uninjured skin from 38 sub-acute burn patients. LDH activity aided the identification of spatially correlating immunocompetent cells in a sub-group of six patients. Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) was used to describe relative lactate and pyruvate abundance in burned and uninjured tissue. LDH activity was significantly increased in the middle and deep regions of burnt skin compared with superficial areas in burnt skin and uninjured tissue and positively correlated with post-burn time. Regions of increased LDH activity showed high pyruvate and low lactate abundance when examined with DESI-MSI. Areas of increased LDH activity exhibited cellular infiltration, including CD3 + and CD4 + T-lymphocytes and CD68 + macrophages. Our data demonstrate a steady increase in functional LDH activity in sub-acute burn wounds linked to cellular infiltration. The cell types associated are related to tissue restructuring and inflammation. This region in burn wounds is likely the focus of dysregulated inflammation and hypermetabolism. Nature Publishing Group UK 2021-10-28 /pmc/articles/PMC8553775/ /pubmed/34711882 http://dx.doi.org/10.1038/s41598-021-00644-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cuddihy, Joshua
Wu, Gongjie
Ho, Laptin
Kudo, Hiromi
Dannhorn, Andreas
Mandalia, Sundhiya
Collins, Declan
Weir, Justin
Spencer, Ashley
Vizcaychipi, Marcela
Takats, Zoltan
Nagy, Istvan
Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title_full Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title_fullStr Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title_full_unstemmed Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title_short Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
title_sort lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553775/
https://www.ncbi.nlm.nih.gov/pubmed/34711882
http://dx.doi.org/10.1038/s41598-021-00644-5
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