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Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

Circular RNA (circRNA) are a recently discovered class of RNA characterized by a covalently-bonded back-splice junction. As circRNAs are inherently more stable than other RNA species, they may be detected extracellularly in peripheral biofluids and provide novel biomarkers. While circRNA have been i...

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Autores principales: Hutchins, Elizabeth, Reiman, Rebecca, Winarta, Joseph, Beecroft, Taylor, Richholt, Ryan, De Both, Matt, Shahbander, Khalouk, Carlson, Elizabeth, Janss, Alex, Siniard, Ashley, Balak, Chris, Bruhns, Ryan, Whitsett, Timothy G., McCoy, Roger, Anastasi, Matthew, Allen, April, Churas, Brian, Huentelman, Matthew, Van Keuren-Jensen, Kendall
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553830/
https://www.ncbi.nlm.nih.gov/pubmed/34711851
http://dx.doi.org/10.1038/s41597-021-01056-w
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author Hutchins, Elizabeth
Reiman, Rebecca
Winarta, Joseph
Beecroft, Taylor
Richholt, Ryan
De Both, Matt
Shahbander, Khalouk
Carlson, Elizabeth
Janss, Alex
Siniard, Ashley
Balak, Chris
Bruhns, Ryan
Whitsett, Timothy G.
McCoy, Roger
Anastasi, Matthew
Allen, April
Churas, Brian
Huentelman, Matthew
Van Keuren-Jensen, Kendall
author_facet Hutchins, Elizabeth
Reiman, Rebecca
Winarta, Joseph
Beecroft, Taylor
Richholt, Ryan
De Both, Matt
Shahbander, Khalouk
Carlson, Elizabeth
Janss, Alex
Siniard, Ashley
Balak, Chris
Bruhns, Ryan
Whitsett, Timothy G.
McCoy, Roger
Anastasi, Matthew
Allen, April
Churas, Brian
Huentelman, Matthew
Van Keuren-Jensen, Kendall
author_sort Hutchins, Elizabeth
collection PubMed
description Circular RNA (circRNA) are a recently discovered class of RNA characterized by a covalently-bonded back-splice junction. As circRNAs are inherently more stable than other RNA species, they may be detected extracellularly in peripheral biofluids and provide novel biomarkers. While circRNA have been identified previously in peripheral biofluids, there are few datasets for circRNA junctions from healthy controls. We collected 134 plasma and 114 urine samples from 54 healthy, male college athlete volunteers, and used RNASeq to determine circRNA content. The intersection of six bioinformatic tools identified 965 high-confidence, characteristic circRNA junctions in plasma and 72 in urine. Highly-expressed circRNA junctions were validated by qRT-PCR. Longitudinal samples were collected from a subset, demonstrating circRNA expression was stable over time. Lastly, the ratio of circular to linear transcripts was higher in plasma than urine. This study provides a valuable resource for characterization of circRNA in plasma and urine from healthy volunteers, one that can be developed and reassessed as researchers probe the circRNA contents of biofluids across physiological changes and disease states.
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spelling pubmed-85538302021-10-29 Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes Hutchins, Elizabeth Reiman, Rebecca Winarta, Joseph Beecroft, Taylor Richholt, Ryan De Both, Matt Shahbander, Khalouk Carlson, Elizabeth Janss, Alex Siniard, Ashley Balak, Chris Bruhns, Ryan Whitsett, Timothy G. McCoy, Roger Anastasi, Matthew Allen, April Churas, Brian Huentelman, Matthew Van Keuren-Jensen, Kendall Sci Data Data Descriptor Circular RNA (circRNA) are a recently discovered class of RNA characterized by a covalently-bonded back-splice junction. As circRNAs are inherently more stable than other RNA species, they may be detected extracellularly in peripheral biofluids and provide novel biomarkers. While circRNA have been identified previously in peripheral biofluids, there are few datasets for circRNA junctions from healthy controls. We collected 134 plasma and 114 urine samples from 54 healthy, male college athlete volunteers, and used RNASeq to determine circRNA content. The intersection of six bioinformatic tools identified 965 high-confidence, characteristic circRNA junctions in plasma and 72 in urine. Highly-expressed circRNA junctions were validated by qRT-PCR. Longitudinal samples were collected from a subset, demonstrating circRNA expression was stable over time. Lastly, the ratio of circular to linear transcripts was higher in plasma than urine. This study provides a valuable resource for characterization of circRNA in plasma and urine from healthy volunteers, one that can be developed and reassessed as researchers probe the circRNA contents of biofluids across physiological changes and disease states. Nature Publishing Group UK 2021-10-28 /pmc/articles/PMC8553830/ /pubmed/34711851 http://dx.doi.org/10.1038/s41597-021-01056-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) applies to the metadata files associated with this article.
spellingShingle Data Descriptor
Hutchins, Elizabeth
Reiman, Rebecca
Winarta, Joseph
Beecroft, Taylor
Richholt, Ryan
De Both, Matt
Shahbander, Khalouk
Carlson, Elizabeth
Janss, Alex
Siniard, Ashley
Balak, Chris
Bruhns, Ryan
Whitsett, Timothy G.
McCoy, Roger
Anastasi, Matthew
Allen, April
Churas, Brian
Huentelman, Matthew
Van Keuren-Jensen, Kendall
Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title_full Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title_fullStr Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title_full_unstemmed Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title_short Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
title_sort extracellular circular rna profiles in plasma and urine of healthy, male college athletes
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553830/
https://www.ncbi.nlm.nih.gov/pubmed/34711851
http://dx.doi.org/10.1038/s41597-021-01056-w
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