Cargando…

Quantification of airborne SARS-CoV-2 genomic particles in different hospital settings

We quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein ge...

Descripción completa

Detalles Bibliográficos
Autores principales: Amato-Lourenço, Luís Fernando, de Souza Xavier Costa, Natália, Dantas, Kátia Cristina, Lombardi, Suzette Cleuza Ferreira Spina, Júnior, Alfredo Mendroni, Lindoso, José Angelo Lauletta, Lima, Felipe Gallego, Carvalho-Oliveira, Regiani, Mauad, Thais
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553841/
https://www.ncbi.nlm.nih.gov/pubmed/34711888
http://dx.doi.org/10.1038/s41598-021-00761-1
Descripción
Sumario:We quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein genes. The E-gene was detected in 5 out of 6 samples at the ICU-COVID-19 ward and in 5 out of 7 samples at the ward-COVID-19. Similarly, in the non-dedicated facilities, the E-gene was detected in 5 out of 6 samples collected in the ICU and 4 out of 7 samples in the ward. In the necropsy room, 6 out of 7 samples were positive for the E-gene. When both wards were compared, the non-COVID ward presented a significantly higher concentration of the E-gene than in the COVID-19 ward (p = 0.003). There was no significant difference in E-gene concentration between the ICU-COVID-19 and the ICU (p = 0.548). Likewise, there was no significant difference among E-gene concentrations found in the autopsy room versus the ICUs and wards (dedicated or not) (p = 0.245). Our results show the widespread presence of aerosol contamination in different hospital units.