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Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

Root hair formation in Arabidopsis thaliana is a well-established model system for epidermal patterning and morphogenesis in plants. Over the last decades, many underlying regulatory genes and well-established networks have been identified by thorough genetic and molecular analysis. In this study, w...

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Autores principales: Mapar, Mona, Chopra, Divykriti, Stephan, Lisa, Schrader, Andrea, Sun, Hequan, Schneeberger, Korbinian, Albani, Maria, Coupland, George, Hülskamp, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554057/
https://www.ncbi.nlm.nih.gov/pubmed/34721494
http://dx.doi.org/10.3389/fpls.2021.767772
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author Mapar, Mona
Chopra, Divykriti
Stephan, Lisa
Schrader, Andrea
Sun, Hequan
Schneeberger, Korbinian
Albani, Maria
Coupland, George
Hülskamp, Martin
author_facet Mapar, Mona
Chopra, Divykriti
Stephan, Lisa
Schrader, Andrea
Sun, Hequan
Schneeberger, Korbinian
Albani, Maria
Coupland, George
Hülskamp, Martin
author_sort Mapar, Mona
collection PubMed
description Root hair formation in Arabidopsis thaliana is a well-established model system for epidermal patterning and morphogenesis in plants. Over the last decades, many underlying regulatory genes and well-established networks have been identified by thorough genetic and molecular analysis. In this study, we used a forward genetic approach to identify genes involved in root hair development in Arabis alpina, a related crucifer species that diverged from A. thaliana approximately 26–40 million years ago. We found all root hair mutant classes known in A. thaliana and identified orthologous regulatory genes by whole-genome or candidate gene sequencing. Our findings indicate that the gene-phenotype relationships regulating root hair development are largely conserved between A. thaliana and A. alpina. Concordantly, a detailed analysis of one mutant with multiple hairs originating from one cell suggested that a mutation in the SUPERCENTIPEDE1 (SCN1) gene is causal for the phenotype and that AaSCN1 is fully functional in A. thaliana. Interestingly, we also found differences in the regulation of root hair differentiation and morphogenesis between the species, and a subset of root hair mutants could not be explained by mutations in orthologs of known genes from A. thaliana. This analysis provides insight into the conservation and divergence of root hair regulation in the Brassicaceae.
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spelling pubmed-85540572021-10-30 Genetic and Molecular Analysis of Root Hair Development in Arabis alpina Mapar, Mona Chopra, Divykriti Stephan, Lisa Schrader, Andrea Sun, Hequan Schneeberger, Korbinian Albani, Maria Coupland, George Hülskamp, Martin Front Plant Sci Plant Science Root hair formation in Arabidopsis thaliana is a well-established model system for epidermal patterning and morphogenesis in plants. Over the last decades, many underlying regulatory genes and well-established networks have been identified by thorough genetic and molecular analysis. In this study, we used a forward genetic approach to identify genes involved in root hair development in Arabis alpina, a related crucifer species that diverged from A. thaliana approximately 26–40 million years ago. We found all root hair mutant classes known in A. thaliana and identified orthologous regulatory genes by whole-genome or candidate gene sequencing. Our findings indicate that the gene-phenotype relationships regulating root hair development are largely conserved between A. thaliana and A. alpina. Concordantly, a detailed analysis of one mutant with multiple hairs originating from one cell suggested that a mutation in the SUPERCENTIPEDE1 (SCN1) gene is causal for the phenotype and that AaSCN1 is fully functional in A. thaliana. Interestingly, we also found differences in the regulation of root hair differentiation and morphogenesis between the species, and a subset of root hair mutants could not be explained by mutations in orthologs of known genes from A. thaliana. This analysis provides insight into the conservation and divergence of root hair regulation in the Brassicaceae. Frontiers Media S.A. 2021-10-15 /pmc/articles/PMC8554057/ /pubmed/34721494 http://dx.doi.org/10.3389/fpls.2021.767772 Text en Copyright © 2021 Mapar, Chopra, Stephan, Schrader, Sun, Schneeberger, Albani, Coupland and Hülskamp. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mapar, Mona
Chopra, Divykriti
Stephan, Lisa
Schrader, Andrea
Sun, Hequan
Schneeberger, Korbinian
Albani, Maria
Coupland, George
Hülskamp, Martin
Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title_full Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title_fullStr Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title_full_unstemmed Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title_short Genetic and Molecular Analysis of Root Hair Development in Arabis alpina
title_sort genetic and molecular analysis of root hair development in arabis alpina
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554057/
https://www.ncbi.nlm.nih.gov/pubmed/34721494
http://dx.doi.org/10.3389/fpls.2021.767772
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