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The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa

Intensive farming is prone to induce large-scale outbreaks of infectious diseases, with increasing use of antibiotics, which deviate from the demand of organic farming. The high mortality rate of chickens infected with Salmonella caused huge economic losses; therefore, the promising safe prevention...

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Autores principales: Zhang, Hang, Wang, Minjuan, Jia, Junpeng, Zhao, Jiayi, Radebe, Stoffel Matjeke, Yu, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554125/
https://www.ncbi.nlm.nih.gov/pubmed/34722703
http://dx.doi.org/10.3389/fvets.2021.740424
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author Zhang, Hang
Wang, Minjuan
Jia, Junpeng
Zhao, Jiayi
Radebe, Stoffel Matjeke
Yu, Qinghua
author_facet Zhang, Hang
Wang, Minjuan
Jia, Junpeng
Zhao, Jiayi
Radebe, Stoffel Matjeke
Yu, Qinghua
author_sort Zhang, Hang
collection PubMed
description Intensive farming is prone to induce large-scale outbreaks of infectious diseases, with increasing use of antibiotics, which deviate from the demand of organic farming. The high mortality rate of chickens infected with Salmonella caused huge economic losses; therefore, the promising safe prevention and treatment measures of Salmonella are in urgent need, such as probiotics. Probiotics are becoming an ideal alternative treatment option besides antibiotics, but the effective chicken probiotic strains with clear protective mechanism against Salmonella remain unclear. In this study, we found Enterococcus faecium YQH2 was effective in preventing Salmonella typhimurium infection in chickens. Salmonella typhimurium induced the loss of body weight, and liver and intestinal morphology damage. The inflammatory factor levels increased and intestinal proliferation inhibited. However, after treatment with Enterococcus faecium YQH2, broilers grew normally, the pathological changes of liver and intestine were reduced, and the colonization of Salmonella in the intestine was improved. Not only that, the length of villi and the depth of crypts were relatively normal, and the levels of inflammatory factors such as IL-1β, TNF-α, and IL-8 were reduced. The number of PCNA cells of Enterococcus faecium YQH2 returned to normal under the action of Salmonella typhimurium infection, which was conducive to the normal proliferation of intestinal epithelial cells. The protective effect of Enterococcus faecium YQH2 may be due to the attribution to the activation of hypoxia and then induced the proliferation of intestinal stem cells to repair the damage of intestinal mucosa under Salmonella typhimurium infection. This study demonstrated that Enterococcus faecium YQH2 was effective in preventing Salmonella typhimurium infection, which could be further used in the chicken health breeding.
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spelling pubmed-85541252021-10-30 The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa Zhang, Hang Wang, Minjuan Jia, Junpeng Zhao, Jiayi Radebe, Stoffel Matjeke Yu, Qinghua Front Vet Sci Veterinary Science Intensive farming is prone to induce large-scale outbreaks of infectious diseases, with increasing use of antibiotics, which deviate from the demand of organic farming. The high mortality rate of chickens infected with Salmonella caused huge economic losses; therefore, the promising safe prevention and treatment measures of Salmonella are in urgent need, such as probiotics. Probiotics are becoming an ideal alternative treatment option besides antibiotics, but the effective chicken probiotic strains with clear protective mechanism against Salmonella remain unclear. In this study, we found Enterococcus faecium YQH2 was effective in preventing Salmonella typhimurium infection in chickens. Salmonella typhimurium induced the loss of body weight, and liver and intestinal morphology damage. The inflammatory factor levels increased and intestinal proliferation inhibited. However, after treatment with Enterococcus faecium YQH2, broilers grew normally, the pathological changes of liver and intestine were reduced, and the colonization of Salmonella in the intestine was improved. Not only that, the length of villi and the depth of crypts were relatively normal, and the levels of inflammatory factors such as IL-1β, TNF-α, and IL-8 were reduced. The number of PCNA cells of Enterococcus faecium YQH2 returned to normal under the action of Salmonella typhimurium infection, which was conducive to the normal proliferation of intestinal epithelial cells. The protective effect of Enterococcus faecium YQH2 may be due to the attribution to the activation of hypoxia and then induced the proliferation of intestinal stem cells to repair the damage of intestinal mucosa under Salmonella typhimurium infection. This study demonstrated that Enterococcus faecium YQH2 was effective in preventing Salmonella typhimurium infection, which could be further used in the chicken health breeding. Frontiers Media S.A. 2021-10-15 /pmc/articles/PMC8554125/ /pubmed/34722703 http://dx.doi.org/10.3389/fvets.2021.740424 Text en Copyright © 2021 Zhang, Wang, Jia, Zhao, Radebe and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhang, Hang
Wang, Minjuan
Jia, Junpeng
Zhao, Jiayi
Radebe, Stoffel Matjeke
Yu, Qinghua
The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title_full The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title_fullStr The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title_full_unstemmed The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title_short The Protective Effect of E. faecium on S. typhimurium Infection Induced Damage to Intestinal Mucosa
title_sort protective effect of e. faecium on s. typhimurium infection induced damage to intestinal mucosa
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554125/
https://www.ncbi.nlm.nih.gov/pubmed/34722703
http://dx.doi.org/10.3389/fvets.2021.740424
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