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Antibiotic resistance genes in layer farms and their correlation with environmental samples
Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stages of laye...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554274/ https://www.ncbi.nlm.nih.gov/pubmed/34695626 http://dx.doi.org/10.1016/j.psj.2021.101485 |
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author | Zhu, Ting Chen, Tao Cao, Zhen Zhong, Shan Wen, Xin Mi, Jiandui Ma, Baohua Zou, Yongde Zhang, Na Liao, Xindi Wang, Yan Wu, Yinbao |
author_facet | Zhu, Ting Chen, Tao Cao, Zhen Zhong, Shan Wen, Xin Mi, Jiandui Ma, Baohua Zou, Yongde Zhang, Na Liao, Xindi Wang, Yan Wu, Yinbao |
author_sort | Zhu, Ting |
collection | PubMed |
description | Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stages of layer breeding, layer manure fertilizer, and soil from 9 laying hen farms in Guangdong Province. The detection rate of antibiotic resistance genes was extremely high at the layer farm in manure (100%), layer manure fertilizer (100%), and soil (> 95%). The log counts of antibiotic resistance genes in layer manure (3.34–11.83 log copies/g) were significantly higher than those in layer manure fertilizer (3.45–9.80 log copies/g) and soil (0–7.69 log copies/g). In layer manure, ermB was the most abundant antibiotic resistance gene, with a concentration of 3.19 × 10(9)– 6.82 × 10(11) copies/g. The average abundances of 5 antibiotic resistance genes were above 10(10) copies/g in the descending order ermB, sul2, tetA, sul1, and strB. The relative abundances of ARGs in layer manure samples from different breeding stages ranked as follows: brooding period (BP), late laying period (LL), growing period (GP), early laying period (EL), and peak laying period (PL). There was no significant correlation between the farm scale and the abundance of antibiotic resistance genes. Moreover, the farther away from the layer farm, the lower the abundance of antibiotic resistance genes in the soil. We also found that compost increases the correlation between antibiotic resistance genes, and the antibiotic resistance genes in soil may be directly derived from layer manure fertilizer instead of manure. Therefore, when applying layer manure fertilizer to cultivated land, the risk of antibiotic resistance genes pollution should be acknowledged, and in-depth research should be conducted on how to remove antibiotic resistance genes from layer manure fertilizer to control the spread of antibiotic resistance genes. |
format | Online Article Text |
id | pubmed-8554274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85542742021-11-05 Antibiotic resistance genes in layer farms and their correlation with environmental samples Zhu, Ting Chen, Tao Cao, Zhen Zhong, Shan Wen, Xin Mi, Jiandui Ma, Baohua Zou, Yongde Zhang, Na Liao, Xindi Wang, Yan Wu, Yinbao Poult Sci MICROBIOLOGY AND FOOD SAFETY Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stages of layer breeding, layer manure fertilizer, and soil from 9 laying hen farms in Guangdong Province. The detection rate of antibiotic resistance genes was extremely high at the layer farm in manure (100%), layer manure fertilizer (100%), and soil (> 95%). The log counts of antibiotic resistance genes in layer manure (3.34–11.83 log copies/g) were significantly higher than those in layer manure fertilizer (3.45–9.80 log copies/g) and soil (0–7.69 log copies/g). In layer manure, ermB was the most abundant antibiotic resistance gene, with a concentration of 3.19 × 10(9)– 6.82 × 10(11) copies/g. The average abundances of 5 antibiotic resistance genes were above 10(10) copies/g in the descending order ermB, sul2, tetA, sul1, and strB. The relative abundances of ARGs in layer manure samples from different breeding stages ranked as follows: brooding period (BP), late laying period (LL), growing period (GP), early laying period (EL), and peak laying period (PL). There was no significant correlation between the farm scale and the abundance of antibiotic resistance genes. Moreover, the farther away from the layer farm, the lower the abundance of antibiotic resistance genes in the soil. We also found that compost increases the correlation between antibiotic resistance genes, and the antibiotic resistance genes in soil may be directly derived from layer manure fertilizer instead of manure. Therefore, when applying layer manure fertilizer to cultivated land, the risk of antibiotic resistance genes pollution should be acknowledged, and in-depth research should be conducted on how to remove antibiotic resistance genes from layer manure fertilizer to control the spread of antibiotic resistance genes. Elsevier 2021-09-16 /pmc/articles/PMC8554274/ /pubmed/34695626 http://dx.doi.org/10.1016/j.psj.2021.101485 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MICROBIOLOGY AND FOOD SAFETY Zhu, Ting Chen, Tao Cao, Zhen Zhong, Shan Wen, Xin Mi, Jiandui Ma, Baohua Zou, Yongde Zhang, Na Liao, Xindi Wang, Yan Wu, Yinbao Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title | Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title_full | Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title_fullStr | Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title_full_unstemmed | Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title_short | Antibiotic resistance genes in layer farms and their correlation with environmental samples |
title_sort | antibiotic resistance genes in layer farms and their correlation with environmental samples |
topic | MICROBIOLOGY AND FOOD SAFETY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554274/ https://www.ncbi.nlm.nih.gov/pubmed/34695626 http://dx.doi.org/10.1016/j.psj.2021.101485 |
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