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Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis

Cyanobacteria can utilize CO(2) or even N(2) to produce a variety of high value-added products efficiently. Plastoquinone (PQ) is an important electron carrier in both of the photosynthetic and respiratory electron transport chain. Although the content of PQ, as well as their redox state, have an im...

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Autores principales: Fan, Jianhua, Zhou, Dongqing, Chen, Cheng, Wu, Ju, Wu, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554343/
https://www.ncbi.nlm.nih.gov/pubmed/34754966
http://dx.doi.org/10.1016/j.synbio.2021.10.004
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author Fan, Jianhua
Zhou, Dongqing
Chen, Cheng
Wu, Ju
Wu, Hui
author_facet Fan, Jianhua
Zhou, Dongqing
Chen, Cheng
Wu, Ju
Wu, Hui
author_sort Fan, Jianhua
collection PubMed
description Cyanobacteria can utilize CO(2) or even N(2) to produce a variety of high value-added products efficiently. Plastoquinone (PQ) is an important electron carrier in both of the photosynthetic and respiratory electron transport chain. Although the content of PQ, as well as their redox state, have an important effect on physiology and metabolism, there are relatively few studies on the synthesis of PQ and its related metabolic regulation mechanism in photosynthetic microorganisms. In this study, the strategies of overexpression of Geranyl diphosphate: 4-hydroxybenzoate geranyltransferase (lepgt) and addition of 4-hydroxybenzoate (4-HB) as the quinone ring precursor were adopted to regulate the biosynthesis of PQ in Synechocystis PCC 6803. Combined with the analysis the photosystem activity, respiration rate and metabolic components, we found the changes of intracellular PQ reprogrammed the metabolism of Synechocystis PCC 6803. The results showed that the overexpression of lepgt reduced PQ content dramatically, by 22.18%. Interestingly, both of the photosynthesis and respiration rate were enhanced. In addition, the intracellular lipid and protein contents were significantly increased. Whereas, the addition of low concentrations of 4-HB enhanced the biosynthesis of PQ, and the intracellular PQ contents were increased by 14.76%–70.86% in different conditions. Addition of 4-HB can regulate the photosystem efficiency and respiration and reprogram the metabolism of Synechocystis PCC 6803 efficiently. In a word, regulating the PQ biosynthesis provided a novel idea for promoting the reprogramming the physiology and metabolism of Synechocystis.
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spelling pubmed-85543432021-11-08 Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis Fan, Jianhua Zhou, Dongqing Chen, Cheng Wu, Ju Wu, Hui Synth Syst Biotechnol Article Cyanobacteria can utilize CO(2) or even N(2) to produce a variety of high value-added products efficiently. Plastoquinone (PQ) is an important electron carrier in both of the photosynthetic and respiratory electron transport chain. Although the content of PQ, as well as their redox state, have an important effect on physiology and metabolism, there are relatively few studies on the synthesis of PQ and its related metabolic regulation mechanism in photosynthetic microorganisms. In this study, the strategies of overexpression of Geranyl diphosphate: 4-hydroxybenzoate geranyltransferase (lepgt) and addition of 4-hydroxybenzoate (4-HB) as the quinone ring precursor were adopted to regulate the biosynthesis of PQ in Synechocystis PCC 6803. Combined with the analysis the photosystem activity, respiration rate and metabolic components, we found the changes of intracellular PQ reprogrammed the metabolism of Synechocystis PCC 6803. The results showed that the overexpression of lepgt reduced PQ content dramatically, by 22.18%. Interestingly, both of the photosynthesis and respiration rate were enhanced. In addition, the intracellular lipid and protein contents were significantly increased. Whereas, the addition of low concentrations of 4-HB enhanced the biosynthesis of PQ, and the intracellular PQ contents were increased by 14.76%–70.86% in different conditions. Addition of 4-HB can regulate the photosystem efficiency and respiration and reprogram the metabolism of Synechocystis PCC 6803 efficiently. In a word, regulating the PQ biosynthesis provided a novel idea for promoting the reprogramming the physiology and metabolism of Synechocystis. KeAi Publishing 2021-10-23 /pmc/articles/PMC8554343/ /pubmed/34754966 http://dx.doi.org/10.1016/j.synbio.2021.10.004 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Fan, Jianhua
Zhou, Dongqing
Chen, Cheng
Wu, Ju
Wu, Hui
Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title_full Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title_fullStr Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title_full_unstemmed Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title_short Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis
title_sort reprogramming the metabolism of synechocystis pcc 6803 by regulating the plastoquinone biosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554343/
https://www.ncbi.nlm.nih.gov/pubmed/34754966
http://dx.doi.org/10.1016/j.synbio.2021.10.004
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