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Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury

INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL...

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Autores principales: Wong, Ker Rui, O’Brien, William T, Sun, Mujun, Yamakawa, Glenn, O’Brien, Terence J, Mychasiuk, Richelle, Shultz, Sandy R, McDonald, Stuart J, Brady, Rhys D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554541/
https://www.ncbi.nlm.nih.gov/pubmed/34720579
http://dx.doi.org/10.1177/11772719211053449
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author Wong, Ker Rui
O’Brien, William T
Sun, Mujun
Yamakawa, Glenn
O’Brien, Terence J
Mychasiuk, Richelle
Shultz, Sandy R
McDonald, Stuart J
Brady, Rhys D
author_facet Wong, Ker Rui
O’Brien, William T
Sun, Mujun
Yamakawa, Glenn
O’Brien, Terence J
Mychasiuk, Richelle
Shultz, Sandy R
McDonald, Stuart J
Brady, Rhys D
author_sort Wong, Ker Rui
collection PubMed
description INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL levels can be used as a biomarker of TBI in the presence of concomitant peripheral injuries. METHODS: Rats were randomly assigned to one of four injury groups: polytrauma (muscle crush + fracture + TBI; n = 11); peripheral injuries (muscle crush + fracture + sham-TBI; n = 12); TBI-only (sham-muscle crush + sham-fracture + TBI; n = 13); and triple-sham (n = 7). At 2-days post-injury, serum levels of NfL were quantified using a Simoa HD-X Analyzer. RESULTS: Compared to triple-sham rats, serum NfL concentrations were higher in rats with peripheral injuries-only, TBI-only, and polytrauma. When compared to peripheral injury-only rats, serum NfL levels were higher in TBI-only and polytrauma rats. No differences were found between TBI-only and polytrauma rats. CONCLUSION: Serum NfL levels did not differ between TBI-only and polytrauma rats, indicating that significant peripheral injuries did not affect the sensitivity and specificity of serum NfL as a biomarker of moderate TBI. However, the finding of elevated serum NfL levels in rats with peripheral injuries in the absence of a TBI suggests that the presence of such injuries may limit the utility of NfL as a biomarker of less severe TBI (eg, concussion).
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spelling pubmed-85545412021-10-30 Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury Wong, Ker Rui O’Brien, William T Sun, Mujun Yamakawa, Glenn O’Brien, Terence J Mychasiuk, Richelle Shultz, Sandy R McDonald, Stuart J Brady, Rhys D Biomark Insights Short Report INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL levels can be used as a biomarker of TBI in the presence of concomitant peripheral injuries. METHODS: Rats were randomly assigned to one of four injury groups: polytrauma (muscle crush + fracture + TBI; n = 11); peripheral injuries (muscle crush + fracture + sham-TBI; n = 12); TBI-only (sham-muscle crush + sham-fracture + TBI; n = 13); and triple-sham (n = 7). At 2-days post-injury, serum levels of NfL were quantified using a Simoa HD-X Analyzer. RESULTS: Compared to triple-sham rats, serum NfL concentrations were higher in rats with peripheral injuries-only, TBI-only, and polytrauma. When compared to peripheral injury-only rats, serum NfL levels were higher in TBI-only and polytrauma rats. No differences were found between TBI-only and polytrauma rats. CONCLUSION: Serum NfL levels did not differ between TBI-only and polytrauma rats, indicating that significant peripheral injuries did not affect the sensitivity and specificity of serum NfL as a biomarker of moderate TBI. However, the finding of elevated serum NfL levels in rats with peripheral injuries in the absence of a TBI suggests that the presence of such injuries may limit the utility of NfL as a biomarker of less severe TBI (eg, concussion). SAGE Publications 2021-10-26 /pmc/articles/PMC8554541/ /pubmed/34720579 http://dx.doi.org/10.1177/11772719211053449 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Short Report
Wong, Ker Rui
O’Brien, William T
Sun, Mujun
Yamakawa, Glenn
O’Brien, Terence J
Mychasiuk, Richelle
Shultz, Sandy R
McDonald, Stuart J
Brady, Rhys D
Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title_full Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title_fullStr Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title_full_unstemmed Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title_short Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
title_sort serum neurofilament light as a biomarker of traumatic brain injury in the presence of concomitant peripheral injury
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554541/
https://www.ncbi.nlm.nih.gov/pubmed/34720579
http://dx.doi.org/10.1177/11772719211053449
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