Cargando…
Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury
INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554541/ https://www.ncbi.nlm.nih.gov/pubmed/34720579 http://dx.doi.org/10.1177/11772719211053449 |
_version_ | 1784591822143619072 |
---|---|
author | Wong, Ker Rui O’Brien, William T Sun, Mujun Yamakawa, Glenn O’Brien, Terence J Mychasiuk, Richelle Shultz, Sandy R McDonald, Stuart J Brady, Rhys D |
author_facet | Wong, Ker Rui O’Brien, William T Sun, Mujun Yamakawa, Glenn O’Brien, Terence J Mychasiuk, Richelle Shultz, Sandy R McDonald, Stuart J Brady, Rhys D |
author_sort | Wong, Ker Rui |
collection | PubMed |
description | INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL levels can be used as a biomarker of TBI in the presence of concomitant peripheral injuries. METHODS: Rats were randomly assigned to one of four injury groups: polytrauma (muscle crush + fracture + TBI; n = 11); peripheral injuries (muscle crush + fracture + sham-TBI; n = 12); TBI-only (sham-muscle crush + sham-fracture + TBI; n = 13); and triple-sham (n = 7). At 2-days post-injury, serum levels of NfL were quantified using a Simoa HD-X Analyzer. RESULTS: Compared to triple-sham rats, serum NfL concentrations were higher in rats with peripheral injuries-only, TBI-only, and polytrauma. When compared to peripheral injury-only rats, serum NfL levels were higher in TBI-only and polytrauma rats. No differences were found between TBI-only and polytrauma rats. CONCLUSION: Serum NfL levels did not differ between TBI-only and polytrauma rats, indicating that significant peripheral injuries did not affect the sensitivity and specificity of serum NfL as a biomarker of moderate TBI. However, the finding of elevated serum NfL levels in rats with peripheral injuries in the absence of a TBI suggests that the presence of such injuries may limit the utility of NfL as a biomarker of less severe TBI (eg, concussion). |
format | Online Article Text |
id | pubmed-8554541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-85545412021-10-30 Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury Wong, Ker Rui O’Brien, William T Sun, Mujun Yamakawa, Glenn O’Brien, Terence J Mychasiuk, Richelle Shultz, Sandy R McDonald, Stuart J Brady, Rhys D Biomark Insights Short Report INTRODUCTION: Serum neurofilament light (NfL) is an emerging biomarker of traumatic brain injury (TBI). However, the effect of peripheral injuries such as long bone fracture and skeletal muscle injury on serum NfL levels is unknown. Therefore, the aim of this study was to determine whether serum NfL levels can be used as a biomarker of TBI in the presence of concomitant peripheral injuries. METHODS: Rats were randomly assigned to one of four injury groups: polytrauma (muscle crush + fracture + TBI; n = 11); peripheral injuries (muscle crush + fracture + sham-TBI; n = 12); TBI-only (sham-muscle crush + sham-fracture + TBI; n = 13); and triple-sham (n = 7). At 2-days post-injury, serum levels of NfL were quantified using a Simoa HD-X Analyzer. RESULTS: Compared to triple-sham rats, serum NfL concentrations were higher in rats with peripheral injuries-only, TBI-only, and polytrauma. When compared to peripheral injury-only rats, serum NfL levels were higher in TBI-only and polytrauma rats. No differences were found between TBI-only and polytrauma rats. CONCLUSION: Serum NfL levels did not differ between TBI-only and polytrauma rats, indicating that significant peripheral injuries did not affect the sensitivity and specificity of serum NfL as a biomarker of moderate TBI. However, the finding of elevated serum NfL levels in rats with peripheral injuries in the absence of a TBI suggests that the presence of such injuries may limit the utility of NfL as a biomarker of less severe TBI (eg, concussion). SAGE Publications 2021-10-26 /pmc/articles/PMC8554541/ /pubmed/34720579 http://dx.doi.org/10.1177/11772719211053449 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Short Report Wong, Ker Rui O’Brien, William T Sun, Mujun Yamakawa, Glenn O’Brien, Terence J Mychasiuk, Richelle Shultz, Sandy R McDonald, Stuart J Brady, Rhys D Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title | Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title_full | Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title_fullStr | Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title_full_unstemmed | Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title_short | Serum Neurofilament Light as a Biomarker of Traumatic Brain Injury in the Presence of Concomitant Peripheral Injury |
title_sort | serum neurofilament light as a biomarker of traumatic brain injury in the presence of concomitant peripheral injury |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554541/ https://www.ncbi.nlm.nih.gov/pubmed/34720579 http://dx.doi.org/10.1177/11772719211053449 |
work_keys_str_mv | AT wongkerrui serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT obrienwilliamt serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT sunmujun serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT yamakawaglenn serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT obrienterencej serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT mychasiukrichelle serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT shultzsandyr serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT mcdonaldstuartj serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury AT bradyrhysd serumneurofilamentlightasabiomarkeroftraumaticbraininjuryinthepresenceofconcomitantperipheralinjury |