Cargando…
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline co...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554630/ https://www.ncbi.nlm.nih.gov/pubmed/34746855 http://dx.doi.org/10.1016/j.xpro.2021.100872 |
_version_ | 1784591834131988480 |
---|---|
author | Boutet-Robinet, Elisa Haykal, Maria M. Hashim, Saleha Frisan, Teresa Martin, Océane C.B. |
author_facet | Boutet-Robinet, Elisa Haykal, Maria M. Hashim, Saleha Frisan, Teresa Martin, Océane C.B. |
author_sort | Boutet-Robinet, Elisa |
collection | PubMed |
description | We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]). For complete details on the use and execution of this protocol, please refer to Martin et al. (2021). |
format | Online Article Text |
id | pubmed-8554630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85546302021-11-05 Detection of DNA damage by alkaline comet assay in mouse colonic mucosa Boutet-Robinet, Elisa Haykal, Maria M. Hashim, Saleha Frisan, Teresa Martin, Océane C.B. STAR Protoc Protocol We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]). For complete details on the use and execution of this protocol, please refer to Martin et al. (2021). Elsevier 2021-10-23 /pmc/articles/PMC8554630/ /pubmed/34746855 http://dx.doi.org/10.1016/j.xpro.2021.100872 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Boutet-Robinet, Elisa Haykal, Maria M. Hashim, Saleha Frisan, Teresa Martin, Océane C.B. Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title_full | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title_fullStr | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title_full_unstemmed | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title_short | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
title_sort | detection of dna damage by alkaline comet assay in mouse colonic mucosa |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554630/ https://www.ncbi.nlm.nih.gov/pubmed/34746855 http://dx.doi.org/10.1016/j.xpro.2021.100872 |
work_keys_str_mv | AT boutetrobinetelisa detectionofdnadamagebyalkalinecometassayinmousecolonicmucosa AT haykalmariam detectionofdnadamagebyalkalinecometassayinmousecolonicmucosa AT hashimsaleha detectionofdnadamagebyalkalinecometassayinmousecolonicmucosa AT frisanteresa detectionofdnadamagebyalkalinecometassayinmousecolonicmucosa AT martinoceanecb detectionofdnadamagebyalkalinecometassayinmousecolonicmucosa |