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Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model

BACKGROUND: Cornea is a composite tissue exhibiting nonlinear and time-dependent mechanical properties. Corneal ulcers are one of the main pathologies that affect this tissue, disrupting its structural integrity and leading to impaired functions. In this study, uniaxial tensile and stress-relaxation...

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Autores principales: Fontanella, Chiara Giulia, Carniel, Emanuele Luigi, Corain, Livio, Peruffo, Antonella, Iacopetti, Ilaria, Pavan, Piero G., Todros, Silvia, Perazzi, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555156/
https://www.ncbi.nlm.nih.gov/pubmed/34711207
http://dx.doi.org/10.1186/s12917-021-03050-1
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author Fontanella, Chiara Giulia
Carniel, Emanuele Luigi
Corain, Livio
Peruffo, Antonella
Iacopetti, Ilaria
Pavan, Piero G.
Todros, Silvia
Perazzi, Anna
author_facet Fontanella, Chiara Giulia
Carniel, Emanuele Luigi
Corain, Livio
Peruffo, Antonella
Iacopetti, Ilaria
Pavan, Piero G.
Todros, Silvia
Perazzi, Anna
author_sort Fontanella, Chiara Giulia
collection PubMed
description BACKGROUND: Cornea is a composite tissue exhibiting nonlinear and time-dependent mechanical properties. Corneal ulcers are one of the main pathologies that affect this tissue, disrupting its structural integrity and leading to impaired functions. In this study, uniaxial tensile and stress-relaxation tests are developed to evaluate stress-strain and time-dependent mechanical behaviour of porcine corneas. RESULTS: The samples are split in two groups: some corneas are analysed in an unaltered state (healthy samples), while others are injured with alkaline solution to create an experimental ulcer (lesioned samples). Furthermore, within each group, corneas are examined in two conditions: few hours after the enucleation (fresh samples) or after 7 days in a specific culture medium for the tissue (cultured samples). Finally, another condition is added: corneas from all the groups undergo or not a cross-linking treatment. In both stress-strain and stress-relaxation tests, a weakening of the tissue is observed due to the imposed conditions (lesion, culture and treatment), represented by a lower stiffness and increased stress-relaxation. CONCLUSIONS: Alkali-induced corneal stromal melting determines changes in the mechanical response that can be related to a damage at microstructural level. The results of the present study represent the basis for the investigation of traditional and innovative corneal therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-03050-1.
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spelling pubmed-85551562021-10-29 Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model Fontanella, Chiara Giulia Carniel, Emanuele Luigi Corain, Livio Peruffo, Antonella Iacopetti, Ilaria Pavan, Piero G. Todros, Silvia Perazzi, Anna BMC Vet Res Research BACKGROUND: Cornea is a composite tissue exhibiting nonlinear and time-dependent mechanical properties. Corneal ulcers are one of the main pathologies that affect this tissue, disrupting its structural integrity and leading to impaired functions. In this study, uniaxial tensile and stress-relaxation tests are developed to evaluate stress-strain and time-dependent mechanical behaviour of porcine corneas. RESULTS: The samples are split in two groups: some corneas are analysed in an unaltered state (healthy samples), while others are injured with alkaline solution to create an experimental ulcer (lesioned samples). Furthermore, within each group, corneas are examined in two conditions: few hours after the enucleation (fresh samples) or after 7 days in a specific culture medium for the tissue (cultured samples). Finally, another condition is added: corneas from all the groups undergo or not a cross-linking treatment. In both stress-strain and stress-relaxation tests, a weakening of the tissue is observed due to the imposed conditions (lesion, culture and treatment), represented by a lower stiffness and increased stress-relaxation. CONCLUSIONS: Alkali-induced corneal stromal melting determines changes in the mechanical response that can be related to a damage at microstructural level. The results of the present study represent the basis for the investigation of traditional and innovative corneal therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-03050-1. BioMed Central 2021-10-28 /pmc/articles/PMC8555156/ /pubmed/34711207 http://dx.doi.org/10.1186/s12917-021-03050-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Fontanella, Chiara Giulia
Carniel, Emanuele Luigi
Corain, Livio
Peruffo, Antonella
Iacopetti, Ilaria
Pavan, Piero G.
Todros, Silvia
Perazzi, Anna
Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title_full Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title_fullStr Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title_full_unstemmed Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title_short Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
title_sort mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555156/
https://www.ncbi.nlm.nih.gov/pubmed/34711207
http://dx.doi.org/10.1186/s12917-021-03050-1
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