Cargando…

Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer

BACKGROUND: Formalin-fixed paraffin embedded (FFPE) tissues may provide an exciting resource to study microbial associations in human disease, but the use of these low biomass specimens remains challenging. We aimed to reduce unintentional bacterial interference in molecular analysis of FFPE tissues...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lam, Suk Yee, Ioannou, Athanasia, Konstanti, Prokopis, Visseren, Thijmen, Doukas, Michail, Peppelenbosch, Maikel Petrus, Belzer, Clara, Fuhler, Gwenny Manel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555202/ https://www.ncbi.nlm.nih.gov/pubmed/34715774 http://dx.doi.org/10.1186/s12866-021-02359-z

Ejemplares similares

Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
por: Robertson, David, et al.
Publicado: (2008)

Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
por: Magdeldin, Sameh, et al.
Publicado: (2012)

Recurrence of primary sclerosing cholangitis after liver transplantation is associated with specific changes in the gut microbiome pretransplant – a pilot study
por: Visseren, Thijmen, et al.
Publicado: (2020)

Transcriptome Profiling of Archived Sectioned Formalin-Fixed Paraffin-Embedded (AS-FFPE) Tissue for Disease Classification
por: Kojima, Kensuke, et al.
Publicado: (2014)

miRNA expression profiling of formalin-fixed paraffin-embedded (FFPE) hereditary breast tumors
por: Tanić, Miljana, et al.
Publicado: (2014)

KRAS Mutation Detection in Paired Frozen and Formalin-Fixed Paraffin-Embedded (FFPE) Colorectal Cancer Tissues
por: Solassol, Jérome, et al.
Publicado: (2011)

Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer Tissue
por: Menon, Roopika, et al.
Publicado: (2012)

Aberrant MicroRNA Expression in Endometrial Carcinoma Using Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
por: Lee, Taek Sang, et al.
Publicado: (2013)

An optimized workflow for microCT imaging of formalin‐fixed and paraffin‐embedded (FFPE) early equine embryos
por: Handschuh, Stephan, et al.
Publicado: (2022)

A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
por: Mitsa, Georgia, et al.
Publicado: (2022)

A novel method for analyzing formalin-fixed paraffin embedded (FFPE) tissue sections by mass spectrometry imaging
por: Aoki, Yutaka, et al.
Publicado: (2007)

Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA
por: Stiller, Mathias, et al.
Publicado: (2016)

Tissue lithography: Microscale dewaxing to enable retrospective studies on formalin-fixed paraffin-embedded (FFPE) tissue sections
por: Cors, Julien F., et al.
Publicado: (2017)

Whole exome sequencing (WES) on formalin-fixed, paraffin-embedded (FFPE) tumor tissue in gastrointestinal stromal tumors (GIST)
por: Astolfi, Annalisa, et al.
Publicado: (2015)

Formalin-Fixed Paraffin-Embedded (FFPE) samples are not a beneficial replacement for frozen tissues in fetal membrane microbiota research
por: Hockney, Rochelle, et al.
Publicado: (2022)

The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies
por: Zhang, Pan, et al.
Publicado: (2017)

Molecular characterisation of formalin-fixed paraffin-embedded (FFPE) breast tumour specimens using a custom 512-gene breast cancer bead array-based platform
por: Abramovitz, M, et al.
Publicado: (2011)

A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies
por: Popp, Alina, et al.
Publicado: (2020)

987. Identification of Fungal Pathogens In Formalin-Fixed Paraffin-Embedded (FFPE) Tissues by Targeted Next-Generation Sequencing (NGS) Technology
por: Totten, Marissa C, et al.
Publicado: (2023)

Transcriptome profiling from formalin-fixed, paraffin-embedded tumor specimens by RNA-seq
por: Qu, Kunbin, et al.
Publicado: (2010)

Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
por: Kalmár, Alexandra, et al.
Publicado: (2015)

Fabrication of Formalin-Fixed, Paraffin-Embedded (FFPE) Circulating Tumor Cell (CTC) Block Using a Hydrogel Core-Mediated Method
por: Lee, Tae Hee, et al.
Publicado: (2021)

Correction to: A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies
por: Popp, Alina, et al.
Publicado: (2020)

SARS-CoV-2 RNA detection in Formalin-Fixed Paraffin-Embedded (FFPE) tissue by droplet digital PCR (ddPCR)
por: Majumdar, Ramanath, et al.
Publicado: (2022)

Cannabinoid Signal Transduction Explains Disconnect of Cannabis Effects in Experimental and Clinical Colitis
por: Peppelenbosch, Maikel P, et al.
Publicado: (2019)

Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens
por: Gouveia, Gisele Rodrigues, et al.
Publicado: (2014)

Comparison of Different Buffers for Protein Extraction from Formalin-Fixed and Paraffin-Embedded Tissue Specimens
por: Shen, Kaini, et al.
Publicado: (2015)

RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining
por: Guo, Yan, et al.
Publicado: (2016)

Discovery of Novel and Clinically Relevant Markers in Formalin-Fixed Paraffin-Embedded Esophageal Cancer Specimen
por: Abdo, Joe, et al.
Publicado: (2018)

Performance of Oncomine Fusion Transcript kit for formalin‐fixed, paraffin‐embedded lung cancer specimens
por: Sakai, Kazuko, et al.
Publicado: (2019)

Pre-Analytical Considerations for Successful Next-Generation Sequencing (NGS): Challenges and Opportunities for Formalin-Fixed and Paraffin-Embedded Tumor Tissue (FFPE) Samples
por: Arreaza, Gladys, et al.
Publicado: (2016)

Gene expression profiling using formalin-fixed paraffin-embedded primary specimens of AIDS-related Lymphomas
por: Hassane, Duane, et al.
Publicado: (2012)

Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis
por: Wakabayashi, Koichi, et al.
Publicado: (2014)

Corrigendum: Discovery of Novel and Clinically Relevant Markers in Formalin-Fixed Paraffin-Embedded Esophageal Cancer Specimen
por: Abdo, Joe, et al.
Publicado: (2019)

Proteomic Analysis of Matched Formalin-Fixed, Paraffin-Embedded Specimens in Patients with Advanced Serous Ovarian Carcinoma
por: Smith, Ashlee L., et al.
Publicado: (2013)

Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin‐fixed paraffin‐embedded specimens
por: Kohsaka, Shinji, et al.
Publicado: (2019)

Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens
por: Sadamoto, Sota, et al.
Publicado: (2022)

A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks
por: Mansour, Anthony, et al.
Publicado: (2014)

Identification and validation of differentially expressed transcripts by RNA-sequencing of formalin-fixed, paraffin-embedded (FFPE) lung tissue from patients with Idiopathic Pulmonary Fibrosis
por: Vukmirovic, Milica, et al.
Publicado: (2017)

Assessment of the quality of DNA from various formalin-fixed paraffin-embedded (FFPE) tissues and the use of this DNA for next-generation sequencing (NGS) with no artifactual mutation
por: Einaga, Naoki, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_2v23uu47925elu1fvck0ofv03r