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Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection

Understanding how exogenous microbes stably colonize the animal gut is essential to reveal mechanisms of action and tailor effective probiotic treatments. Bifidobacterium species are naturally enriched in the gastrointestinal tract of breast-fed infants. Human milk oligosaccharides (HMOs) are associ...

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Autores principales: Heiss, Britta E., Ehrlich, Amy M., Maldonado-Gomez, Maria X., Taft, Diana H., Larke, Jules A., Goodson, Michael L., Slupsky, Carolyn M., Tancredi, Daniel J., Raybould, Helen E., Mills, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555557/
https://www.ncbi.nlm.nih.gov/pubmed/34705611
http://dx.doi.org/10.1080/19490976.2021.1986666
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author Heiss, Britta E.
Ehrlich, Amy M.
Maldonado-Gomez, Maria X.
Taft, Diana H.
Larke, Jules A.
Goodson, Michael L.
Slupsky, Carolyn M.
Tancredi, Daniel J.
Raybould, Helen E.
Mills, David A.
author_facet Heiss, Britta E.
Ehrlich, Amy M.
Maldonado-Gomez, Maria X.
Taft, Diana H.
Larke, Jules A.
Goodson, Michael L.
Slupsky, Carolyn M.
Tancredi, Daniel J.
Raybould, Helen E.
Mills, David A.
author_sort Heiss, Britta E.
collection PubMed
description Understanding how exogenous microbes stably colonize the animal gut is essential to reveal mechanisms of action and tailor effective probiotic treatments. Bifidobacterium species are naturally enriched in the gastrointestinal tract of breast-fed infants. Human milk oligosaccharides (HMOs) are associated with this enrichment. However, direct mechanistic proof of the importance of HMOs in this colonization is lacking given milk contains additional factors that impact the gut microbiota. This study examined mice supplemented with the HMO 2ʹfucosyllactose (2ʹFL) together with a 2ʹFL-consuming strain, Bifidobacterium pseudocatenulatum MP80. 2ʹFL supplementation creates a niche for high levels of B.p. MP80 persistence, similar to Bifidobacterium levels seen in breast-fed infants. This synergism impacted gut microbiota composition, activated anti-inflammatory pathways and protected against chemically-induced colitis. These results demonstrate that bacterial-milk glycan interactions alone drive enrichment of beneficial Bifidobacterium and provide a model for tunable colonization thus facilitating insight into mechanisms of health promotion by bifidobacteriain neonates.
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spelling pubmed-85555572021-10-30 Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection Heiss, Britta E. Ehrlich, Amy M. Maldonado-Gomez, Maria X. Taft, Diana H. Larke, Jules A. Goodson, Michael L. Slupsky, Carolyn M. Tancredi, Daniel J. Raybould, Helen E. Mills, David A. Gut Microbes Research Paper Understanding how exogenous microbes stably colonize the animal gut is essential to reveal mechanisms of action and tailor effective probiotic treatments. Bifidobacterium species are naturally enriched in the gastrointestinal tract of breast-fed infants. Human milk oligosaccharides (HMOs) are associated with this enrichment. However, direct mechanistic proof of the importance of HMOs in this colonization is lacking given milk contains additional factors that impact the gut microbiota. This study examined mice supplemented with the HMO 2ʹfucosyllactose (2ʹFL) together with a 2ʹFL-consuming strain, Bifidobacterium pseudocatenulatum MP80. 2ʹFL supplementation creates a niche for high levels of B.p. MP80 persistence, similar to Bifidobacterium levels seen in breast-fed infants. This synergism impacted gut microbiota composition, activated anti-inflammatory pathways and protected against chemically-induced colitis. These results demonstrate that bacterial-milk glycan interactions alone drive enrichment of beneficial Bifidobacterium and provide a model for tunable colonization thus facilitating insight into mechanisms of health promotion by bifidobacteriain neonates. Taylor & Francis 2021-10-27 /pmc/articles/PMC8555557/ /pubmed/34705611 http://dx.doi.org/10.1080/19490976.2021.1986666 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Heiss, Britta E.
Ehrlich, Amy M.
Maldonado-Gomez, Maria X.
Taft, Diana H.
Larke, Jules A.
Goodson, Michael L.
Slupsky, Carolyn M.
Tancredi, Daniel J.
Raybould, Helen E.
Mills, David A.
Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title_full Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title_fullStr Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title_full_unstemmed Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title_short Bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
title_sort bifidobacterium catabolism of human milk oligosaccharides overrides endogenous competitive exclusion driving colonization and protection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555557/
https://www.ncbi.nlm.nih.gov/pubmed/34705611
http://dx.doi.org/10.1080/19490976.2021.1986666
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