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Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development

Ribonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their...

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Autores principales: Sankaranarayanan, M., Emenecker, Ryan J., Wilby, Elise L., Jahnel, Marcus, Trussina, Irmela R.E.A., Wayland, Matt, Alberti, Simon, Holehouse, Alex S., Weil, Timothy T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555633/
https://www.ncbi.nlm.nih.gov/pubmed/34655524
http://dx.doi.org/10.1016/j.devcel.2021.09.021
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author Sankaranarayanan, M.
Emenecker, Ryan J.
Wilby, Elise L.
Jahnel, Marcus
Trussina, Irmela R.E.A.
Wayland, Matt
Alberti, Simon
Holehouse, Alex S.
Weil, Timothy T.
author_facet Sankaranarayanan, M.
Emenecker, Ryan J.
Wilby, Elise L.
Jahnel, Marcus
Trussina, Irmela R.E.A.
Wayland, Matt
Alberti, Simon
Holehouse, Alex S.
Weil, Timothy T.
author_sort Sankaranarayanan, M.
collection PubMed
description Ribonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their impact on mRNA storage in mature Drosophila oocytes. We show that the conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested physical state. We demonstrate that structurally distinct proteins and protein-protein interactions, together with RNA, regulate the physical properties of P bodies. Using live imaging and in situ hybridization, we show that the arrested state and integrity of P bodies support the storage of bicoid (bcd) mRNA and that egg activation modulates P body properties, leading to the release of bcd for translation in the early embryo. Together, this work provides an example of how physical states of condensates regulate cellular function in development.
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spelling pubmed-85556332021-11-08 Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development Sankaranarayanan, M. Emenecker, Ryan J. Wilby, Elise L. Jahnel, Marcus Trussina, Irmela R.E.A. Wayland, Matt Alberti, Simon Holehouse, Alex S. Weil, Timothy T. Dev Cell Article Ribonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their impact on mRNA storage in mature Drosophila oocytes. We show that the conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested physical state. We demonstrate that structurally distinct proteins and protein-protein interactions, together with RNA, regulate the physical properties of P bodies. Using live imaging and in situ hybridization, we show that the arrested state and integrity of P bodies support the storage of bicoid (bcd) mRNA and that egg activation modulates P body properties, leading to the release of bcd for translation in the early embryo. Together, this work provides an example of how physical states of condensates regulate cellular function in development. Cell Press 2021-10-25 /pmc/articles/PMC8555633/ /pubmed/34655524 http://dx.doi.org/10.1016/j.devcel.2021.09.021 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sankaranarayanan, M.
Emenecker, Ryan J.
Wilby, Elise L.
Jahnel, Marcus
Trussina, Irmela R.E.A.
Wayland, Matt
Alberti, Simon
Holehouse, Alex S.
Weil, Timothy T.
Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title_full Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title_fullStr Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title_full_unstemmed Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title_short Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
title_sort adaptable p body physical states differentially regulate bicoid mrna storage during early drosophila development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555633/
https://www.ncbi.nlm.nih.gov/pubmed/34655524
http://dx.doi.org/10.1016/j.devcel.2021.09.021
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