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Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models

Electrical impedance myography (EIM) using surface techniques has shown promise as a means of diagnosing and tracking disorders affecting muscle and assessing treatment efficacy. However, the relationship between such surface-obtained impedance values and pure muscle impedance values has not been es...

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Autores principales: Pandeya, Sarbesh R., Nagy, Janice A., Riveros, Daniela, Semple, Carson, Taylor, Rebecca S., Sanchez, Benjamin, Rutkove, Seward B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555802/
https://www.ncbi.nlm.nih.gov/pubmed/34714853
http://dx.doi.org/10.1371/journal.pone.0259071
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author Pandeya, Sarbesh R.
Nagy, Janice A.
Riveros, Daniela
Semple, Carson
Taylor, Rebecca S.
Sanchez, Benjamin
Rutkove, Seward B.
author_facet Pandeya, Sarbesh R.
Nagy, Janice A.
Riveros, Daniela
Semple, Carson
Taylor, Rebecca S.
Sanchez, Benjamin
Rutkove, Seward B.
author_sort Pandeya, Sarbesh R.
collection PubMed
description Electrical impedance myography (EIM) using surface techniques has shown promise as a means of diagnosing and tracking disorders affecting muscle and assessing treatment efficacy. However, the relationship between such surface-obtained impedance values and pure muscle impedance values has not been established. Here we studied three groups of diseased and wild-type (WT) animals, including a Duchenne muscular dystrophy model (the D2-mdx mouse), an amyotrophic lateral sclerosis (ALS) model (the SOD1 G93A mouse), and a model of fat-related atrophy (the db/db diabetic obese mouse), performing hind limb measurements using a standard surface array and ex vivo measurements on freshly excised gastrocnemius muscle. A total of 101 animals (23 D2-mdx, 43 ALS mice, 12 db/db mice, and corresponding 30 WT mice) were studied with EIM across a frequency range of 8 kHz to 1 MHz. For both D2-mdx and ALS models, moderate strength correlations (Spearman rho values generally ranging from 0.3–0.7, depending on the impedance parameter (i.e., resistance, reactance and phase) were obtained. In these groups of animals, there was an offset in frequency with impedance values obtained at higher surface frequencies correlating more strongly to impedance values obtained at lower ex vivo frequencies. For the db/db model, correlations were comparatively weaker and strongest at very high and very low frequencies. When combining impedance data from all three disease models together, moderate correlations persisted (with maximal Spearman rho values of 0.45). These data support that surface EIM data reflect ex vivo muscle tissue EIM values to a moderate degree across several different diseases, with the highest correlations occurring in the 10–200 kHz frequency range. Understanding these relationships will prove useful for future applications of the technique of EIM in the assessment of neuromuscular disorders.
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spelling pubmed-85558022021-10-30 Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models Pandeya, Sarbesh R. Nagy, Janice A. Riveros, Daniela Semple, Carson Taylor, Rebecca S. Sanchez, Benjamin Rutkove, Seward B. PLoS One Research Article Electrical impedance myography (EIM) using surface techniques has shown promise as a means of diagnosing and tracking disorders affecting muscle and assessing treatment efficacy. However, the relationship between such surface-obtained impedance values and pure muscle impedance values has not been established. Here we studied three groups of diseased and wild-type (WT) animals, including a Duchenne muscular dystrophy model (the D2-mdx mouse), an amyotrophic lateral sclerosis (ALS) model (the SOD1 G93A mouse), and a model of fat-related atrophy (the db/db diabetic obese mouse), performing hind limb measurements using a standard surface array and ex vivo measurements on freshly excised gastrocnemius muscle. A total of 101 animals (23 D2-mdx, 43 ALS mice, 12 db/db mice, and corresponding 30 WT mice) were studied with EIM across a frequency range of 8 kHz to 1 MHz. For both D2-mdx and ALS models, moderate strength correlations (Spearman rho values generally ranging from 0.3–0.7, depending on the impedance parameter (i.e., resistance, reactance and phase) were obtained. In these groups of animals, there was an offset in frequency with impedance values obtained at higher surface frequencies correlating more strongly to impedance values obtained at lower ex vivo frequencies. For the db/db model, correlations were comparatively weaker and strongest at very high and very low frequencies. When combining impedance data from all three disease models together, moderate correlations persisted (with maximal Spearman rho values of 0.45). These data support that surface EIM data reflect ex vivo muscle tissue EIM values to a moderate degree across several different diseases, with the highest correlations occurring in the 10–200 kHz frequency range. Understanding these relationships will prove useful for future applications of the technique of EIM in the assessment of neuromuscular disorders. Public Library of Science 2021-10-29 /pmc/articles/PMC8555802/ /pubmed/34714853 http://dx.doi.org/10.1371/journal.pone.0259071 Text en © 2021 Pandeya et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pandeya, Sarbesh R.
Nagy, Janice A.
Riveros, Daniela
Semple, Carson
Taylor, Rebecca S.
Sanchez, Benjamin
Rutkove, Seward B.
Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title_full Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title_fullStr Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title_full_unstemmed Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title_short Relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
title_sort relationships between in vivo surface and ex vivo electrical impedance myography measurements in three different neuromuscular disorder mouse models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555802/
https://www.ncbi.nlm.nih.gov/pubmed/34714853
http://dx.doi.org/10.1371/journal.pone.0259071
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