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Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants
The photosynthetic performance of crop plants under a variety of environmental factors and stress conditions, at the fundamental level, depends largely on the organization and structural flexibility of thylakoid membranes. These highly organized membranes accommodate virtually all protein complexes...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556207/ https://www.ncbi.nlm.nih.gov/pubmed/32488447 http://dx.doi.org/10.1007/s11120-020-00763-6 |
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author | Nagy, Gergely Garab, Győző |
author_facet | Nagy, Gergely Garab, Győző |
author_sort | Nagy, Gergely |
collection | PubMed |
description | The photosynthetic performance of crop plants under a variety of environmental factors and stress conditions, at the fundamental level, depends largely on the organization and structural flexibility of thylakoid membranes. These highly organized membranes accommodate virtually all protein complexes and additional compounds carrying out the light reactions of photosynthesis. Most regulatory mechanisms fine-tuning the photosynthetic functions affect the organization of thylakoid membranes at different levels of the structural complexity. In order to monitor these reorganizations, non-invasive techniques are of special value. On the mesoscopic scale, small-angle neutron scattering (SANS) has been shown to deliver statistically and spatially averaged information on the periodic organization of the thylakoid membranes in vivo and/or, in isolated thylakoids, under physiologically relevant conditions, without fixation or staining. More importantly, SANS investigations have revealed rapid reversible reorganizations on the timescale of several seconds and minutes. In this paper, we give a short introduction into the basics of SANS technique, advantages and limitations, and briefly overview recent advances and potential applications of this technique in the physiology and biotechnology of crop plants. We also discuss future perspectives of neutron crystallography and different neutron scattering techniques, which are anticipated to become more accessible and of more use in photosynthesis research at new facilities with higher fluxes and innovative instrumentation. |
format | Online Article Text |
id | pubmed-8556207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-85562072021-11-04 Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants Nagy, Gergely Garab, Győző Photosynth Res Original Article The photosynthetic performance of crop plants under a variety of environmental factors and stress conditions, at the fundamental level, depends largely on the organization and structural flexibility of thylakoid membranes. These highly organized membranes accommodate virtually all protein complexes and additional compounds carrying out the light reactions of photosynthesis. Most regulatory mechanisms fine-tuning the photosynthetic functions affect the organization of thylakoid membranes at different levels of the structural complexity. In order to monitor these reorganizations, non-invasive techniques are of special value. On the mesoscopic scale, small-angle neutron scattering (SANS) has been shown to deliver statistically and spatially averaged information on the periodic organization of the thylakoid membranes in vivo and/or, in isolated thylakoids, under physiologically relevant conditions, without fixation or staining. More importantly, SANS investigations have revealed rapid reversible reorganizations on the timescale of several seconds and minutes. In this paper, we give a short introduction into the basics of SANS technique, advantages and limitations, and briefly overview recent advances and potential applications of this technique in the physiology and biotechnology of crop plants. We also discuss future perspectives of neutron crystallography and different neutron scattering techniques, which are anticipated to become more accessible and of more use in photosynthesis research at new facilities with higher fluxes and innovative instrumentation. Springer Netherlands 2020-06-02 2021 /pmc/articles/PMC8556207/ /pubmed/32488447 http://dx.doi.org/10.1007/s11120-020-00763-6 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Nagy, Gergely Garab, Győző Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title | Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title_full | Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title_fullStr | Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title_full_unstemmed | Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title_short | Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
title_sort | neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556207/ https://www.ncbi.nlm.nih.gov/pubmed/32488447 http://dx.doi.org/10.1007/s11120-020-00763-6 |
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