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Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells
Rotating forms of suspension culture allow cells to aggregate into spheroids, prevent the de-differentiating influence of 2D culture, and, perhaps most importantly of all, provide physiologically relevant, in vivo levels of shear stress. Rotating suspension culture technology has not been widely imp...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556299/ https://www.ncbi.nlm.nih.gov/pubmed/34716334 http://dx.doi.org/10.1038/s41598-021-00304-8 |
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author | Hammond, Timothy G. Nislow, Corey Christov, Ivan C. Batuman, Vecihi Nagrani, Pranay P. Barazandeh, Marjan Upadhyay, Rohit Giaever, Guri Allen, Patricia L. Armbruster, Michael Raymond, Allen Birdsall, Holly H. |
author_facet | Hammond, Timothy G. Nislow, Corey Christov, Ivan C. Batuman, Vecihi Nagrani, Pranay P. Barazandeh, Marjan Upadhyay, Rohit Giaever, Guri Allen, Patricia L. Armbruster, Michael Raymond, Allen Birdsall, Holly H. |
author_sort | Hammond, Timothy G. |
collection | PubMed |
description | Rotating forms of suspension culture allow cells to aggregate into spheroids, prevent the de-differentiating influence of 2D culture, and, perhaps most importantly of all, provide physiologically relevant, in vivo levels of shear stress. Rotating suspension culture technology has not been widely implemented, in large part because the vessels are prohibitively expensive, labor-intensive to use, and are difficult to scale for industrial applications. Our solution addresses each of these challenges in a new vessel called a cell spinpod. These small 3.5 mL capacity vessels are constructed from injection-molded thermoplastic polymer components. They contain self-sealing axial silicone rubber ports, and fluoropolymer, breathable membranes. Here we report the two-fluid modeling of the flow and stresses in cell spinpods. Cell spinpods were used to demonstrate the effect of fluid shear stress on renal cell gene expression and cellular functions, particularly membrane and xenobiotic transporters, mitochondrial function, and myeloma light chain, cisplatin and doxorubicin, toxicity. During exposure to myeloma immunoglobulin light chains, rotation increased release of clinically validated nephrotoxicity cytokine markers in a toxin-specific pattern. Addition of cisplatin or doxorubicin nephrotoxins reversed the enhanced glucose and albumin uptake induced by fluid shear stress in rotating cell spinpod cultures. Cell spinpods are a simple, inexpensive, easily automated culture device that enhances cellular functions for in vitro studies of nephrotoxicity. |
format | Online Article Text |
id | pubmed-8556299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85562992021-11-01 Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells Hammond, Timothy G. Nislow, Corey Christov, Ivan C. Batuman, Vecihi Nagrani, Pranay P. Barazandeh, Marjan Upadhyay, Rohit Giaever, Guri Allen, Patricia L. Armbruster, Michael Raymond, Allen Birdsall, Holly H. Sci Rep Article Rotating forms of suspension culture allow cells to aggregate into spheroids, prevent the de-differentiating influence of 2D culture, and, perhaps most importantly of all, provide physiologically relevant, in vivo levels of shear stress. Rotating suspension culture technology has not been widely implemented, in large part because the vessels are prohibitively expensive, labor-intensive to use, and are difficult to scale for industrial applications. Our solution addresses each of these challenges in a new vessel called a cell spinpod. These small 3.5 mL capacity vessels are constructed from injection-molded thermoplastic polymer components. They contain self-sealing axial silicone rubber ports, and fluoropolymer, breathable membranes. Here we report the two-fluid modeling of the flow and stresses in cell spinpods. Cell spinpods were used to demonstrate the effect of fluid shear stress on renal cell gene expression and cellular functions, particularly membrane and xenobiotic transporters, mitochondrial function, and myeloma light chain, cisplatin and doxorubicin, toxicity. During exposure to myeloma immunoglobulin light chains, rotation increased release of clinically validated nephrotoxicity cytokine markers in a toxin-specific pattern. Addition of cisplatin or doxorubicin nephrotoxins reversed the enhanced glucose and albumin uptake induced by fluid shear stress in rotating cell spinpod cultures. Cell spinpods are a simple, inexpensive, easily automated culture device that enhances cellular functions for in vitro studies of nephrotoxicity. Nature Publishing Group UK 2021-10-29 /pmc/articles/PMC8556299/ /pubmed/34716334 http://dx.doi.org/10.1038/s41598-021-00304-8 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hammond, Timothy G. Nislow, Corey Christov, Ivan C. Batuman, Vecihi Nagrani, Pranay P. Barazandeh, Marjan Upadhyay, Rohit Giaever, Guri Allen, Patricia L. Armbruster, Michael Raymond, Allen Birdsall, Holly H. Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title | Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title_full | Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title_fullStr | Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title_full_unstemmed | Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title_short | Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
title_sort | cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556299/ https://www.ncbi.nlm.nih.gov/pubmed/34716334 http://dx.doi.org/10.1038/s41598-021-00304-8 |
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