Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice

Neuronal loss resulting from progressive neurodegeneration is a major pathological feature of Alzheimer’s disease (AD). Here, we present a protocol to detect neurodegeneration, neuronal apoptosis, and neuronal loss in 5XFAD mouse strain, which is a well-established model for interrogating the molecu...

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Detalles Bibliográficos
Autores principales: Zhang, Liansheng, Li, Jie, Lin, Anning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556527/
https://www.ncbi.nlm.nih.gov/pubmed/34755118
http://dx.doi.org/10.1016/j.xpro.2021.100915
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author Zhang, Liansheng
Li, Jie
Lin, Anning
author_facet Zhang, Liansheng
Li, Jie
Lin, Anning
author_sort Zhang, Liansheng
collection PubMed
description Neuronal loss resulting from progressive neurodegeneration is a major pathological feature of Alzheimer’s disease (AD). Here, we present a protocol to detect neurodegeneration, neuronal apoptosis, and neuronal loss in 5XFAD mouse strain, which is a well-established model for interrogating the molecular mechanism of neuronal death in AD. This protocol describes the use of the neurodegenerative marker Fluro-Jade C, cleaved caspase-3 immunofluorescent staining and Nissl staining for the analysis of neurodegeneration and neuronal loss in 5XFAD mice. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
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spelling pubmed-85565272021-11-08 Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice Zhang, Liansheng Li, Jie Lin, Anning STAR Protoc Protocol Neuronal loss resulting from progressive neurodegeneration is a major pathological feature of Alzheimer’s disease (AD). Here, we present a protocol to detect neurodegeneration, neuronal apoptosis, and neuronal loss in 5XFAD mouse strain, which is a well-established model for interrogating the molecular mechanism of neuronal death in AD. This protocol describes the use of the neurodegenerative marker Fluro-Jade C, cleaved caspase-3 immunofluorescent staining and Nissl staining for the analysis of neurodegeneration and neuronal loss in 5XFAD mice. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021). Elsevier 2021-10-25 /pmc/articles/PMC8556527/ /pubmed/34755118 http://dx.doi.org/10.1016/j.xpro.2021.100915 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Liansheng
Li, Jie
Lin, Anning
Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title_full Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title_fullStr Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title_full_unstemmed Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title_short Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice
title_sort assessment of neurodegeneration and neuronal loss in aged 5xfad mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556527/
https://www.ncbi.nlm.nih.gov/pubmed/34755118
http://dx.doi.org/10.1016/j.xpro.2021.100915
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AT lijie assessmentofneurodegenerationandneuronallossinaged5xfadmice
AT linanning assessmentofneurodegenerationandneuronallossinaged5xfadmice

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