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Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia

INTRODUCTION: Understanding the epidemiology of tuberculosis is limited by lack of genotyping data. We sought to characterize the drug susceptibility testing patterns and genetic diversity of M. tuberculosis isolates in southern Ethiopia. METHODOLOGY: A cross-sectional study was conducted among newl...

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Autores principales: Merid, Yared, Hailu, Elena, Habtamu, Getnet, Tilahun, Melaku, Abebe, Markos, Hailu, Mesay, Hailu, Tsegaye, Datiko, Daniel G, Woldeamanuel, Yimtubezinash, Aseffa, Abraham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556644/
https://www.ncbi.nlm.nih.gov/pubmed/34669600
http://dx.doi.org/10.3855/jidc.14742
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author Merid, Yared
Hailu, Elena
Habtamu, Getnet
Tilahun, Melaku
Abebe, Markos
Hailu, Mesay
Hailu, Tsegaye
Datiko, Daniel G
Woldeamanuel, Yimtubezinash
Aseffa, Abraham
author_facet Merid, Yared
Hailu, Elena
Habtamu, Getnet
Tilahun, Melaku
Abebe, Markos
Hailu, Mesay
Hailu, Tsegaye
Datiko, Daniel G
Woldeamanuel, Yimtubezinash
Aseffa, Abraham
author_sort Merid, Yared
collection PubMed
description INTRODUCTION: Understanding the epidemiology of tuberculosis is limited by lack of genotyping data. We sought to characterize the drug susceptibility testing patterns and genetic diversity of M. tuberculosis isolates in southern Ethiopia. METHODOLOGY: A cross-sectional study was conducted among newly diagnosed sputum smear positive patients with tuberculosis visiting nine health facilities in southern Ethiopia from June 2015 to May 2016. Three consecutive sputum samples (spot-morning-spot) per patient were examined using acid-fast bacilli smear microscopy with all smear positive specimens having acid-fast bacilli cultures performed. M. tuberculosis isolates had drug susceptibility testing performed using indirect proportion method and were genotyped with RD9 deletion analysis and spoligotyping. Mapping of strain was made using geographic information system. RESULTS: Among 250 newly diagnosed patients with tuberculosis, 4% were HIV co-infected. All 230 isolates tested were M. tuberculosis strains belonging to three lineages: Euro-American, 187 (81%), East-African-Indian, 31 (14%), and Lineage 7 (Ethiopian lineage), 8 (4%); categorized into 63 different spoligotype patterns, of which 85% fell into 28 clusters. M. tuberculosis strains were clustered by geographic localities. The dominant spoligotypes were SIT149 (21%) and SIT53 (19%). Drug susceptibility testing found that 14% of isolates tested were resistant to ≥ 1 first line anti- tuberculosis drugs and 11% to INH. SIT 149 was dominant among drug resistant isolates. CONCLUSIONS: The study revealed several clusters and drug resistant strains of M. tuberculosis in the study area, suggesting recent transmission including of drug resistant tuberculosis. Wider monitoring of drug susceptibility testing and geospatial analysis of transmission trends is required to control tuberculosis in southern Ethiopia.
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spelling pubmed-85566442021-10-30 Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia Merid, Yared Hailu, Elena Habtamu, Getnet Tilahun, Melaku Abebe, Markos Hailu, Mesay Hailu, Tsegaye Datiko, Daniel G Woldeamanuel, Yimtubezinash Aseffa, Abraham J Infect Dev Ctries Article INTRODUCTION: Understanding the epidemiology of tuberculosis is limited by lack of genotyping data. We sought to characterize the drug susceptibility testing patterns and genetic diversity of M. tuberculosis isolates in southern Ethiopia. METHODOLOGY: A cross-sectional study was conducted among newly diagnosed sputum smear positive patients with tuberculosis visiting nine health facilities in southern Ethiopia from June 2015 to May 2016. Three consecutive sputum samples (spot-morning-spot) per patient were examined using acid-fast bacilli smear microscopy with all smear positive specimens having acid-fast bacilli cultures performed. M. tuberculosis isolates had drug susceptibility testing performed using indirect proportion method and were genotyped with RD9 deletion analysis and spoligotyping. Mapping of strain was made using geographic information system. RESULTS: Among 250 newly diagnosed patients with tuberculosis, 4% were HIV co-infected. All 230 isolates tested were M. tuberculosis strains belonging to three lineages: Euro-American, 187 (81%), East-African-Indian, 31 (14%), and Lineage 7 (Ethiopian lineage), 8 (4%); categorized into 63 different spoligotype patterns, of which 85% fell into 28 clusters. M. tuberculosis strains were clustered by geographic localities. The dominant spoligotypes were SIT149 (21%) and SIT53 (19%). Drug susceptibility testing found that 14% of isolates tested were resistant to ≥ 1 first line anti- tuberculosis drugs and 11% to INH. SIT 149 was dominant among drug resistant isolates. CONCLUSIONS: The study revealed several clusters and drug resistant strains of M. tuberculosis in the study area, suggesting recent transmission including of drug resistant tuberculosis. Wider monitoring of drug susceptibility testing and geospatial analysis of transmission trends is required to control tuberculosis in southern Ethiopia. 2021-09-30 /pmc/articles/PMC8556644/ /pubmed/34669600 http://dx.doi.org/10.3855/jidc.14742 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Merid, Yared
Hailu, Elena
Habtamu, Getnet
Tilahun, Melaku
Abebe, Markos
Hailu, Mesay
Hailu, Tsegaye
Datiko, Daniel G
Woldeamanuel, Yimtubezinash
Aseffa, Abraham
Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title_full Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title_fullStr Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title_full_unstemmed Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title_short Molecular Epidemiology of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south Ethiopia
title_sort molecular epidemiology of mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in south ethiopia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556644/
https://www.ncbi.nlm.nih.gov/pubmed/34669600
http://dx.doi.org/10.3855/jidc.14742
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